Dynactin 1 Antikörper (C-Term)
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- Target Alle Dynactin 1 (DCTN1) Antikörper anzeigen
- Dynactin 1 (DCTN1)
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Bindungsspezifität
- C-Term
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Reaktivität
- Human
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Wirt
- Ziege
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Klonalität
- Polyklonal
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Konjugat
- Dieser Dynactin 1 Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)
- Verwendungszweck
- Dynactin
- Sequenz
- QEQLHQLHSR LIS
- Spezifität
- This antibody is expected to recognise all reported isoforms.
- Kreuzreaktivität
- Human, Maus
- Aufreinigung
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Güteklasse
- Verified
- Immunogen
- Peptide with sequence C-QEQLHQLHSRLIS, from the C Terminus of the protein sequence according to NP_004073.2, NP_075408.1, NP_001128512.1, NP_001128513.1.
- Isotyp
- IgG
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- Applikationshinweise
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Immunohistochemistry: Paraffin embedded Human Brain (Cortex). Recommended concentration: 6-7 μg/mL.
Western Blot: Approx. 150 kDa band observed in HeLa and MCF7 cell lysates, and in preliminary testing of HEK293 and Mouse Brain lysate (calculated MW of 142 kDa according to NP_004073.2). Recommended concentration 0.3-1 μg/mL. Primary incubation 1 hour at
Peptide ELISA: antibody detection limit dilution 1:16000.
- Kommentare
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Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa cells and additionally the nucleus of U2OS cells.. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of HeL
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- 0.5 mg/mL
- Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handhabung
- Minimize freezing and thawing.
- Lagerung
- -20 °C
- Informationen zur Lagerung
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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Temporal changes in expression of connexin 43 after load-induced hypertrophy in vitro." in: American journal of physiology. Heart and circulatory physiology, Vol. 296, Issue 3, pp. H806-14, (2009) (PubMed).
: "
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Temporal changes in expression of connexin 43 after load-induced hypertrophy in vitro." in: American journal of physiology. Heart and circulatory physiology, Vol. 296, Issue 3, pp. H806-14, (2009) (PubMed).
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- Target
- Dynactin 1 (DCTN1)
- Andere Bezeichnung
- DCTN1 (DCTN1 Produkte)
- Synonyme
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DAP-150 antikoerper, DP-150 antikoerper, P135 antikoerper, Chip150 antikoerper, DCTN1 antikoerper, DKFZp469C1813 antikoerper, AL022633 antikoerper, Glued antikoerper, p150 antikoerper, p150
antikoerper, CG9206 antikoerper, DYNA_DROME antikoerper, Dmel\\CG9206 antikoerper, FCP-D antikoerper, Glu antikoerper, P150 antikoerper, P150[Glued] antikoerper, p150/Glued antikoerper, p150/glued antikoerper, p150[Glued] antikoerper, p150[glued] antikoerper, t1 antikoerper, Dynactin-1 antikoerper, glued antikoerper, dynactin subunit 1 antikoerper, dynactin 1 antikoerper, Dynactin 1, p150 subunit antikoerper, dynactin subunit 1 L homeolog antikoerper, DCTN1 antikoerper, Dctn1 antikoerper, DCTN1-p150 antikoerper, dctn1.L antikoerper - Hintergrund
- DCTN1, dynactin 1 (p150, glued homolog, Drosophila), P135, DP-150, DAP-150, p150-glued, p150, Glued (Drosophila) homolog, 150 kDa dynein-associated polypeptide, dynactin 1 (p150, Glued (Drosophila) homolog), HMN7B, OTTHUMP00000202491, dynactin 1, p150, Gl
- Gen-ID
- 1639, 13191
- NCBI Accession
- NP_004073, NP_075408, NP_001128512, NP_001128513, NP_001177765, NP_001177766
- Pathways
- M Phase, ER-Nucleus Signaling
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