NUP85 Antikörper (C-Term)

Produktnummer ABIN185140

Produktdetails anti-NUP85 Antikörper

Target: NUP85 (Nucleoporin 85kDa (NUP85))

Bindungsspezifität: C-Term

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser NUP85 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Verwendungszweck: Pericentrin 1 / NUP85

Sequenz: ARAIIREGSL EGS

Spezifität: Please note that, in contrast to other anti-Pericentrin antibodies available, this ab is NOT expected to recognize Kendrin (also known as Pericentrin 2). This may explain why it recognizes a 75KDa band (the expected size according to NP_079120) and not a

Kreuzreaktivität: Human, Maus, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Güteklasse: Verified

Immunogen: C-ARAIIREGSLEGS

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Western Blot: Approx 75 kDa band observed in HeLa lysates (calculated MW of 75 kDa according to NP_079120.1). Recommended concentration: 1-3 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:32000.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Referenzen für anti-NUP85 Antikörper (ABIN185140)

Toda, Terashima, Sato, Hirose, Kanegasaki, Matsushima: "FROUNT is a common regulator of CCR2 and CCR5 signaling to control directional migration." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 183, Issue 10, pp. 6387-94, (2009) (PubMed).

Details zu NUP85

Target: NUP85 (Nucleoporin 85kDa (NUP85))

Andere Bezeichnung: NUP85 (NUP85 Produkte)

Hintergrund: NUP85, pericentrin 1, PCNT1, PCN, PCNT, frount, nucleoporin Nup75, nucleoporin 85 kDa, FLJ12549, Nup75, nucleoporin 85

Gen-ID: 79902, 18541

NCBI Accession: NP_079120