PARD6A Antikörper (C-Term)

Produktnummer ABIN185152

Produktdetails anti-PARD6A Antikörper

Target: PARD6A (Par-6 Partitioning Defective 6 Homolog alpha (PARD6A))

Bindungsspezifität: C-Term

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser PARD6A Antikörper ist unkonjugiert

Applikation: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Verwendungszweck: PAR6alpha / PARD6A

Sequenz: GSRIRGDGSG FSL

Spezifität: This antibody is expected to recognise both reported isoforms (NP_058644.1, NP_001032358.1).

Kreuzreaktivität: Human

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Güteklasse: Verified

Immunogen: Peptide with sequence C-GSRIRGDGSGFSL, from the C Terminus of the protein sequence according to NP_058644.1, NP_001032358.1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: In paraffin embedded Human Pancreas, shows strong cytoplasmic staining in rare cells of intralobular ducts. Recommended concentration: 10 μg/mL. Also tested in paraffin embedded Human Heart where it shows speckled staining on myocar

Western Blot: Approx 40 kDa band observed in lysates of cell lines Jurkat and glioblastoma U251 (calculated MW of 37.4 kDa according to NP_058644.1) . Recommended concentration: 1-3 μg/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:8000.

Kommentare:

Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concentration: 10ug/ml.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu PARD6A

Target: PARD6A (Par-6 Partitioning Defective 6 Homolog alpha (PARD6A))

Andere Bezeichnung: PARD6A (PARD6A Produkte)

Synonyme: PARD6A antikoerper, 0710008C04Rik antikoerper, 2610010A15Rik antikoerper, PAR6alpha antikoerper, Par-6 antikoerper, Par6 antikoerper, Par6c antikoerper, TAX40 antikoerper, Tip-40 antikoerper, Par-6a antikoerper, Par6a antikoerper, PAR-6A antikoerper, PAR6 antikoerper, PAR6C antikoerper, TIP-40 antikoerper, par-6 partitioning defective 6 homolog alpha (C. elegans) antikoerper, par-6 family cell polarity regulator alpha antikoerper, Partitioning defective protein 6 antikoerper, PARD6A antikoerper, Pard6a antikoerper, par-6 antikoerper

Hintergrund: PARD6A, PAR6alpha, PAR-6, TAX40, PAR-6A, TIP-40, par-6 partitioning defective 6 homolog alpha (C.elegans), Tax interaction protein 40, par-6 (partitioning defective 6, C.elegans) homolog alpha, PAR6, PAR6C, Tax-interacting protein 40, par-6 partitioning d

Gen-ID: 50855

NCBI Accession: NP_058644, NP_001032358

Pathways: Cell-Cell Junction Organization