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STK35 Antikörper (C-Term)

Verified STK35 Reaktivität: Human, Maus, Schwein WB, ELISA, IHC, IF, FACS Wirt: Ziege Polyclonal unconjugated
Produktnummer ABIN185178
  • Target Alle STK35 Antikörper anzeigen
    STK35 (Serine/threonine Kinase 35 (STK35))
    Bindungsspezifität
    • 15
    • 7
    • 6
    • 2
    • 1
    • 1
    • 1
    • 1
    C-Term
    Reaktivität
    • 26
    • 17
    • 11
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Human, Maus, Schwein
    Wirt
    • 41
    Ziege
    Klonalität
    • 41
    Polyklonal
    Konjugat
    • 12
    • 4
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser STK35 Antikörper ist unkonjugiert
    Applikation
    • 30
    • 15
    • 13
    • 13
    • 9
    • 5
    • 3
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)
    Verwendungszweck
    Clik1 / STK35
    Sequenz
    ELETRMDQVT CAA
    Kreuzreaktivität
    Rind (Kuh), Hund, Human, Maus, Schwein, Ratte
    Aufreinigung
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Güteklasse
    Verified
    Immunogen
    Peptide with sequence ELETRMDQVTCAA, from the C Terminus of the protein sequence according to NP_543026.2.
    Isotyp
    IgG
  • Applikationshinweise

    Immunohistochemistry: In paraffin embedded Human Testis shows granular cytoplasmic staining of Leydig cells. Recommended concentration: 10 μg/mL.

    Western Blot: Approx 55 kDa band observed in Human Breast cancer and in Mouse and Pig Testis lysates, and approx. 58 kDa in nuclear lysates of cell line HEK293 (calculated MW of 58.1 kDa according to Human NP_543026.2, 59.5 kDa according to Mouse NP_899085.3

    Peptide ELISA: antibody detection limit dilution 1:16000.

    Kommentare

    Immunofluorescence: Strong expression of the protein seen in the nuclei of U2OS cells. Recommended concentration: 10μg/ml.

    Flow Cytometry: Flow cytometric analysis of HEK293 cells. Recommended concentration: 10ug/ml.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handhabung
    Minimize freezing and thawing.
    Lagerung
    -20 °C
    Informationen zur Lagerung
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Goyal, Behring, Kumar, Siess: "Identifying and characterizing a novel protein kinase STK35L1 and deciphering its orthologs and close-homologs in vertebrates." in: PLoS ONE, Vol. 4, Issue 9, pp. e6981, (2009) (PubMed).

  • Target
    STK35 (Serine/threonine Kinase 35 (STK35))
    Andere Bezeichnung
    STK35 (STK35 Produkte)
    Synonyme
    clik1 antikoerper, stk35l1 antikoerper, MGC69346 antikoerper, im:6905214 antikoerper, zgc:153287 antikoerper, MGC115406 antikoerper, CLIK1 antikoerper, STK35L1 antikoerper, 1700054C12Rik antikoerper, Stk35l1 antikoerper, serine/threonine kinase 35 antikoerper, serine/threonine kinase 35 S homeolog antikoerper, stk35 antikoerper, stk35.S antikoerper, STK35 antikoerper, Stk35 antikoerper
    Hintergrund
    STK35, Clik1, bA550O8.2, bA550O8.2 CLIK1, serine/threonine kinase 35, CLP-36 interacting kinase, CLIK1, STK35L1, OTTHUMP00000030006, serine threonine kinase 35 long form
    Gen-ID
    140901, 67333
    NCBI Accession
    NP_543026
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