Androgen Receptor Antikörper (N-Term)

Produktnummer ABIN185266

Produktdetails anti-Androgen Receptor Antikörper

Target: Androgen Receptor (AR)

Bindungsspezifität: N-Term

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Androgen Receptor Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP)

Verwendungszweck: Androgen Receptor

Sequenz: EVQLGLGRVY PRPPSC

Spezifität: This antibody is expected to recognize isoform 1 (NP_000035.2) only.

Homologie: Human, Mouse, Rat, Dog, Pig, Cow

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Güteklasse: Verified

Immunogen: Peptide with sequence EVQLGLGRVYPRPPSC, from the N Terminus of the protein sequence according to NP_000035.2.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

DS WB Results: Approx 100 kDa band observed in Human Cerebellum lysates (calculated MW of 99.2 kDa according to NP_000035.2). Recommended concentration:0.1-0.5 μg/mL. Primary incubation 1 hour at room temperature. Negative Control: Human Pancreas lysate. Preliminary testing was unsuccessful on Mouse and Rat for this particular batch.

DS IHC Results: In paraffin embedded Human Prostate shows nuclear staining in the secretory cells of the gland. Recommended concentration: 2-3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:64000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the mitochondria/cytoplasm of MCF7 and U2OS cells. Recommended concentration: 10μg/ml.

Chromatin Immunoprecipitation: A six-fold increased presence of the FKBP5 locus observed when comparing the precipitations using EB06441 with non-specific goat IgG to bring down Androgen Receptor in a chromatin lysate from DHT-stimulated HEC50 cells.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu Androgen Receptor

Target: Androgen Receptor (AR)

Andere Bezeichnung: Androgen Receptor (AR Produkte)

Synonyme: AIS antikoerper, DHTR antikoerper, HUMARA antikoerper, HYSP1 antikoerper, KD antikoerper, NR3C4 antikoerper, SBMA antikoerper, SMAX1 antikoerper, TFM antikoerper, AW320017 antikoerper, Tfm antikoerper, Andr antikoerper, AR antikoerper, androgen receptor antikoerper, AR antikoerper, Ar antikoerper, ar antikoerper

Hintergrund: AR, androgen receptor, dihydrotestosterone receptor, KD, AIS, TFM, DHTR, SBMA, NR3C4, SMAX1, HUMARA, HYSP1, androgen receptor, dihydrotestosterone receptor, RP11-383C12.1

Gen-ID: 367

NCBI Accession: NP_000035

Pathways: Nuclear Receptor Transcription Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Nuclear Hormone Receptor Binding, Chromatin Binding