AHI1 Antikörper (N-Term)

Produktnummer ABIN185303

Produktdetails anti-AHI1 Antikörper

Target: AHI1 (Abelson Helper Integration Site 1 (AHI1))

Bindungsspezifität: N-Term

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser AHI1 Antikörper ist unkonjugiert

Applikation: ELISA, Flow Cytometry (FACS)

Verwendungszweck: AHI1 / Jouberin

Sequenz: PTAESEAKVK TC

Homologie: Human

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Güteklasse: Verified

Immunogen: Peptide with sequence PTAESEAKVKTC, from the N Terminus of the protein sequence according to NP_060121.3, NP_001128303.1, NP_001128302.1, NP_001128304.1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

DS WB Results: Preliminary experiments gave a consistent band at approx 75-80 kDa in Human Cerebellum and Frontal Cortex lysates after 0.5 μg/mL antibody staining and was successfully blocked by incubation with the immunizing peptide. Please note that currently we cannot find an explanation in the literature for this band, given the calculated size of 137 kDa according to NP_060121.3

Peptide ELISA: antibody detection limit dilution 1:16000.

Kommentare:

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Referenzen für anti-AHI1 Antikörper (ABIN185303)

Kennah, Ringrose, Zhou, Esmailzadeh, Qian, Su, Zhou, Jiang: "Identification of tyrosine kinase, HCK, and tumor suppressor, BIN1, as potential mediators of AHI-1 oncogene in primary and transformed CTCL cells." in: Blood, Vol. 113, Issue 19, pp. 4646-55, (2009) (PubMed).

Zhou, Zhao, Ringrose, DeGeer, Kennah, Lin, Sheng, Li, Turhan, Jiang: "AHI-1 interacts with BCR-ABL and modulates BCR-ABL transforming activity and imatinib response of CML stem/progenitor cells." in: The Journal of experimental medicine, Vol. 205, Issue 11, pp. 2657-71, (2008) (PubMed).

Details zu AHI1

Target: AHI1 (Abelson Helper Integration Site 1 (AHI1))

Andere Bezeichnung: AHI1 (AHI1 Produkte)

Synonyme: AHI1 antikoerper, AHI-1 antikoerper, JBTS3 antikoerper, ORF1 antikoerper, dJ71N10.1 antikoerper, 1700015F03Rik antikoerper, Ahi-1 antikoerper, D10Bwg0629e antikoerper, Abelson helper integration site 1 antikoerper, AHI1 antikoerper, ahi1 antikoerper, Ahi1 antikoerper

Hintergrund: AHI1, AHI-1, DKFZp686J1653, FLJ14023, FLJ20069, JBTS3, ORF1, dJ71N10.1, Abelson helper integration site, jouberin, Ahi-1 isoform I, OTTHUMP00000017263, OTTHUMP00000017264, OTTHUMP00000017265, contatins SH3 and WD40 domains, Abelson helper integration site 1

Gen-ID: 54806

NCBI Accession: NP_060121, NP_001128304, NP_001337433