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PDE5A Antikörper (C-Term)

Verified PDE5A Reaktivität: Human WB, ELISA, IF, FACS Wirt: Ziege Polyclonal unconjugated
Produktnummer ABIN185350
  • Target Alle PDE5A Antikörper anzeigen
    PDE5A (phosphodiesterase 5A, cGMP-Specific (PDE5A))
    Bindungsspezifität
    • 5
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    C-Term
    Reaktivität
    • 25
    • 9
    • 7
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Wirt
    • 22
    • 3
    Ziege
    Klonalität
    • 23
    • 2
    Polyklonal
    Konjugat
    • 15
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser PDE5A Antikörper ist unkonjugiert
    Applikation
    • 18
    • 12
    • 9
    • 8
    • 6
    • 6
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)
    Verwendungszweck
    PDE5A
    Sequenz
    LINGESGQAK RN
    Spezifität
    This antibody is expected to recognise reported isoforms NP_001074.2, NP_236914.2 and NP_246273.2.
    Kreuzreaktivität
    Rind (Kuh), Hund, Human, Ratte
    Aufreinigung
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Güteklasse
    Verified
    Immunogen
    Peptide with sequence C-LINGESGQAKRN, from the C Terminus of the protein sequence according to NP_001074.2, NP_246273.2, NP_236914.2, (NP_237223).
    Isotyp
    IgG
    Top Product
    Discover our top product PDE5A Primärantikörper
  • Applikationshinweise

    Western Blot: In transfected HEK293 transiently expressing PDE5A isoform 2 a band of approx. 95 kDa is observed. This band is not observed in the non-transfected HEK293 (calculated MW of 94.8 kDa according to isoform 2, NP_236914.2). Recommended concentrat

    Peptide ELISA: antibody detection limit dilution 1:16000.

    Kommentare

    Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa cells. Recommended concentration: 10μg/ml.

    Flow Cytometry: Flow cytometric analysis of HEK293 cells. Recommended concentration: 10ug/ml

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handhabung
    Minimize freezing and thawing.
    Lagerung
    -20 °C
    Informationen zur Lagerung
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Target
    PDE5A (phosphodiesterase 5A, cGMP-Specific (PDE5A))
    Andere Bezeichnung
    PDE5A (PDE5A Produkte)
    Synonyme
    CGB-PDE antikoerper, CN5A antikoerper, PDE5 antikoerper, PDE5A2 antikoerper, Cgbpde antikoerper, Cn5n antikoerper, Pde5 antikoerper, Pde5a1 antikoerper, PDE5A antikoerper, pde5a antikoerper, cgb-pde antikoerper, cn5a antikoerper, pde5 antikoerper, pde5a1 antikoerper, phosphodiesterase 5A antikoerper, phosphodiesterase 5A, cGMP-specific antikoerper, phosphodiesterase 5A S homeolog antikoerper, phosphodiesterase 5A, cGMP-specific, b antikoerper, PDE5A antikoerper, Pde5a antikoerper, pde5a.S antikoerper, pde5ab antikoerper, pde5a antikoerper
    Hintergrund
    PDE5A, CN5A, PDE5, PDE5A1, CGB-PDE, phosphodiesterase 5A, cGMP-specific, cGMP-binding cGMP-specific 3',5'-cyclic nucleotide phosphodiesterase
    Gen-ID
    8654
    NCBI Accession
    NP_001074, NP_246273, NP_236914
    Pathways
    Regulation of G-Protein Coupled Receptor Protein Signaling
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