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Acetylcholinesterase Antikörper (C-Term)

Verified AChE Reaktivität: Human WB, ELISA, IF, FACS Wirt: Ziege Polyclonal unconjugated
Produktnummer ABIN185357
  • Target Alle Acetylcholinesterase (AChE) Antikörper anzeigen
    Acetylcholinesterase (AChE)
    Bindungsspezifität
    • 15
    • 5
    • 5
    • 5
    • 5
    • 4
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term
    Reaktivität
    • 49
    • 32
    • 32
    • 16
    • 9
    • 7
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Human
    Wirt
    • 85
    • 11
    • 6
    • 1
    Ziege
    Klonalität
    • 93
    • 10
    Polyklonal
    Konjugat
    • 53
    • 20
    • 8
    • 5
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser Acetylcholinesterase Antikörper ist unkonjugiert
    Applikation
    • 80
    • 46
    • 22
    • 13
    • 13
    • 13
    • 11
    • 9
    • 8
    • 6
    • 5
    • 5
    • 4
    • 4
    • 2
    • 2
    • 1
    Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)
    Verwendungszweck
    ACHE
    Sequenz
    QFDHYSKQDR CSDL
    Spezifität
    This antibody is expected to recognise isoform NP_000656.1 only (the ubiquitously expressed, hydrophillic form).
    Kreuzreaktivität
    Human, Maus, Ratte
    Aufreinigung
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Güteklasse
    Verified
    Immunogen
    Peptide with sequence QFDHYSKQDRCSDL, from the C Terminus of the protein sequence according to NP_000656.1.
    Isotyp
    IgG
  • Applikationshinweise

    Western Blot: Approx 70 kDa band observed in HepG2 and Jurkat cell lysates (calculated MW of 67.8 kDa according to NP_000656.1). Recommended concentration: 0.3-1 μg/mL. Primary incubation 1 hour at room temperature.

    Peptide ELISA: antibody detection limit dilution 1:32000.

    Kommentare

    Immunofluorescence: Strong expression of the protein seen in the cytoplasm, plasma membrane and nucleus of U2OS cells. Recommended concentration: 10μg/ml.

    Flow Cytometry: Flow cytometric analysis of HeLa cells. Recomm

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handhabung
    Minimize freezing and thawing.
    Lagerung
    -20 °C
    Informationen zur Lagerung
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Target
    Acetylcholinesterase (AChE)
    Andere Bezeichnung
    ACHE (AChE Produkte)
    Hintergrund
    ACHE, YT, acetylcholinesterase (YT blood group), ARACHE, N-ACHE, OTTHUMP00000211347, OTTHUMP00000211349, OTTHUMP00000211356, acetylcholinesterase, apoptosis-related acetylcholinesterase
    Gen-ID
    43
    NCBI Accession
    NP_000656, NP_001269378
    Pathways
    Skeletal Muscle Fiber Development
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