MYD88 Antikörper (Internal Region)

Produktnummer ABIN185362

Produktdetails anti-MYD88 Antikörper

Target: MYD88 (Myeloid Differentiation Primary Response Gene (88) (MYD88))

Bindungsspezifität: Internal Region

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser MYD88 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Verwendungszweck: MYD88

Sequenz: IKYKAMKKEF P

Spezifität: This antibody is expected to recognize reported isoforms 1, 2, 3 and 9.

Kreuzreaktivität: Hund, Human, Maus, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Güteklasse: Verified

Immunogen: Peptide with sequence C-IKYKAMKKEFP, from the internal region of the protein sequence according to NP_002459.1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: Paraffin embedded Human Tonsil. Recommended concentration: 4-6 μg/mL.

Western Blot: Approx 28 kDa band observed in Human Spleen lysates (calculated MW of 28.3 kDa according to NP_001166039.2). Recommended concentration: 0.3-0.5 μg/mL. Primary incubation1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:128000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in U2OS and Jurkat cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concentration: 10ug/ml.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu MYD88

Target: MYD88 (Myeloid Differentiation Primary Response Gene (88) (MYD88))

Andere Bezeichnung: MYD88 (MYD88 Produkte)

Hintergrund: MYD88, myeloid differentiation primary response gene (88), MYD88D, myeloid differentiation primary response gene 88

Molekulargewicht: 33.2kDa

Gen-ID: 4615, 17874, 301059

NCBI Accession: NP_001166038, NP_002459, NP_001166039

Pathways: NF-kappaB Signalweg, TLR Signalweg, Neurotrophin Signalübertragung, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Toll-Like Receptors Cascades