RBP1 Antikörper (C-Term)
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- Target Alle RBP1 Antikörper anzeigen
- RBP1 (Retinol Binding Protein 1, Cellular (RBP1))
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Bindungsspezifität
- C-Term
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Reaktivität
- Human, Maus
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Wirt
- Ziege
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Klonalität
- Polyklonal
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Konjugat
- Dieser RBP1 Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), Immunofluorescence (IF), ELISA, Flow Cytometry (FACS)
- Verwendungszweck
- RBP1
- Sequenz
- VEGVVCKQVF KKVQ
- Kreuzreaktivität
- Rind (Kuh), Human, Maus
- Aufreinigung
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Güteklasse
- Verified
- Immunogen
- Peptide with sequence VEGVVCKQVFKKVQ, from the C Terminus of the protein sequence according to NP_002890.1.
- Isotyp
- IgG
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- Applikationshinweise
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Western Blot: Approx 16 kDa band observed in NIH3T3 and approx 17 kDa in U251 cell lysates. This molecular weight is routinely observed by other sources. (calculated MW of 22.3 kDa according to Human NP_002890.2 and 15.8 kDa according to Mouse NP_035384.1).
Peptide ELISA: antibody detection limit dilution 1:4000.
- Kommentare
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Immunofluorescence: Expression of the protein seen in the cytoplasm and nucleus of U2OS cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10u
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- 0.5 mg/mL
- Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handhabung
- Minimize freezing and thawing.
- Lagerung
- -20 °C
- Informationen zur Lagerung
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- Target
- RBP1 (Retinol Binding Protein 1, Cellular (RBP1))
- Andere Bezeichnung
- RBP1 (RBP1 Produkte)
- Hintergrund
- RBP1, CRBP, RBPC, CRBP1, CRABP-I, retinol binding protein 1, cellular, retinol-binding protein 1, cellular, CRBPI
- Gen-ID
- 5947
- NCBI Accession
- NP_002890
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