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ERK1 Antikörper (N-Term)

Name: ERK1 Antikörper (N-Term)
Brand: antibodies-online
SKU: ABIN185399
Price: 792.57 EUR
Availability: InStock

Verified MAPK3 Reaktivität: Human WB, ELISA, IF, FACS Wirt: Ziege Polyclonal unconjugated

ABIN185399 (0. (ERK1 Antikörper (N-Term))

ABIN185399 Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0. (ERK1 Antikörper (N-Term))

FACS

ABIN185399 (1 μg/mL) staining of A431 (A) and (0. (ERK1 Antikörper (N-Term))

ABIN185399 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0. (ERK1 Antikörper (N-Term))

ABIN185399 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0. (ERK1 Antikörper (N-Term))

6 Abbildungen

Produktnummer ABIN185399

Datenblatt (PDF)

Target Alle ERK1 (MAPK3) Antikörper anzeigen

ERK1 (MAPK3) (Mitogen-Activated Protein Kinase 3 (MAPK3))
Bindungsspezifität
67

43

21

16

15

15

11

11

9

9

8

6

5

5

5

5

3

3

3

3

3

3

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
N-Term
Reaktivität
213

160

133

44

20

19

16

12

10

9

9

7

6

6

5

4

3

3

1

1

1

1
Human
Wirt
218

28

3

1
Ziege
Passende Sekundärantikörper
Klonalität
198

52
Polyklonal
Konjugat
104

17

16

13

7

7

5

5

5

5

5

5

5

5

5

5

5

3

3

3

3

3

3

2

2

2

2

2

2

1
Dieser ERK1 Antikörper ist unkonjugiert
Applikation
199

75

68

65

65

41

39

35

31

21

18

11

7

5

3

2

1

1
Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Verwendungszweck

MAPK3/ERK1

Sequenz

GGEPRRTEGV GP

Spezifität

No cross-reactivity expected with ERK2

Kreuzreaktivität

Human

Aufreinigung

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Güteklasse

Verified

Immunogen

Peptide with sequence GGEPRRTEGVGP-C, from the N Terminus of the protein sequence according to NP_002737.2, NP_001035145.1, NP_001103361.1.

Isotyp

IgG

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Applikationshinweise

Western Blot: Approx 40 kDa band observed in Human Cerebellum lysates and in lysates of cell lines A431 and HepG2, and approx 42 kDa obseved in Human Frontal Cortex lysates (calculated MW of 40.1 kDa according to NP_001035145.1 and 43.1 kDa according to NP_0
Peptide ELISA: antibody detection limit dilution 1:4000.

Kommentare

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa and A431 cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration:

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Liquid

Konzentration

0.5 mg/mL

Buffer

Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel

Sodium azide

Vorsichtsmaßnahmen

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung

Minimize freezing and thawing.

Lagerung

-20 °C

Informationen zur Lagerung

Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
Target

ERK1 (MAPK3) (Mitogen-Activated Protein Kinase 3 (MAPK3))

Andere Bezeichnung

MAPK3 (MAPK3 Produkte)

Hintergrund

MAPK3, ERK1, mitogen-activated protein kinase 3, HGNC:6877, P44ERK1, P44MAPK, PRKM3, protein kinase, mitogen-activated 3 (MAP kinase 3 p44), HS44KDAP, HUMKER1A, MGC20180, OTTHUMP00000174538, extracellular signal-regulated kinase 1, extracellular signal-re

Gen-ID

5595

NCBI Accession

NP_002737, NP_001035145, NP_001103361

Pathways

MAPK Signalweg, RTK Signalweg, Interferon-gamma Pathway, Fc-epsilon Rezeptor Signalübertragung, Neurotrophin Signalübertragung, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, S100 Proteine