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DARC Antikörper (N-Term)

Verified DARC Reaktivität: Human WB, ELISA, IHC, FACS Wirt: Ziege Polyclonal unconjugated
Produktnummer ABIN185480
  • Target Alle DARC Antikörper anzeigen
    DARC (Duffy Blood Group, Chemokine Receptor (DARC))
    Bindungsspezifität
    • 12
    • 12
    • 6
    • 6
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    N-Term
    Reaktivität
    • 39
    • 10
    • 6
    Human
    Wirt
    • 31
    • 8
    • 2
    • 1
    Ziege
    Klonalität
    • 35
    • 6
    • 1
    Polyklonal
    Konjugat
    • 24
    • 4
    • 4
    • 4
    • 3
    • 3
    Dieser DARC Antikörper ist unkonjugiert
    Applikation
    • 28
    • 24
    • 8
    • 6
    • 6
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)
    Verwendungszweck
    Duffy / FY / DARC
    Sequenz
    HRAELSPSTE NSSQLDFED
    Spezifität
    This antibody is expected to recognize both reported isoforms (NP_001116423.1, NP_002027.2).
    Kreuzreaktivität
    Human
    Aufreinigung
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Güteklasse
    Verified
    Immunogen
    Peptide with sequence HRAELSPSTENSSQLDFED-C, from the N Terminus of the protein sequence according to NP_001116423.1, NP_002027.2.
    Isotyp
    IgG
  • Applikationshinweise

    Immunohistochemistry: In paraffin embedded Human Kidney stains epithelial cells of renal tubules and endothelial cells of vessels. The granular staining is compatible with the endoplasmic reticulum association of the protein. Recommended concentration: 3

    Western Blot: Approx 37 kDa band observed in Human Liver lysates (calculated MW of 35.6 kDa according to NP_002027.2). Recommended concentration: 0.3-1 μg/mL. Primary incubation was 1 hour.

    Peptide ELISA: antibody detection limit dilution 1:16000.

    Kommentare

    Flow cytometry: Flow cytometric analysis of HEK293 cells. Recommended concentration: 10ug/ml. A customer has reported successful staining of HEK293S-TetR transfected with DARC receptor at a dilution of 1/1000.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handhabung
    Minimize freezing and thawing.
    Lagerung
    -20 °C
    Informationen zur Lagerung
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Target
    DARC (Duffy Blood Group, Chemokine Receptor (DARC))
    Andere Bezeichnung
    ACKR1 (DARC Produkte)
    Synonyme
    DARC antikoerper, FY antikoerper, ACKR1 antikoerper, CCBP1 antikoerper, CD234 antikoerper, Dfy antikoerper, GPD antikoerper, GpFy antikoerper, WBCQ1 antikoerper, AA162249 antikoerper, Ackr1 antikoerper, ESTM35 antikoerper, Duffy blood group, chemokine receptor antikoerper, atypical chemokine receptor 1 (Duffy blood group) antikoerper, atypical chemokine receptor 1 antikoerper, DARC antikoerper, ACKR1 antikoerper, Ackr1 antikoerper
    Hintergrund
    FY, DARC, HGNC:4035, Duffy blood group, CCBP1, GPD, CTA-134P22.3, CD234, FY, OTTHUMP00000060081, Duffy blood group, chemokine receptor, Duffy blood group antigen, Glycoprotein D, Dfy, GpFy, WBCQ1, Fy glycoprotein, OTTHUMP00000035293, OTTHUMP00000035294
    Gen-ID
    2532
    NCBI Accession
    NP_001116423, NP_002027
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