DARC Antikörper (N-Term)

Produktnummer ABIN185480

Produktdetails anti-DARC Antikörper

Target: DARC (Duffy Blood Group, Chemokine Receptor (DARC))

Bindungsspezifität: N-Term

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser DARC Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Verwendungszweck: Duffy / FY / DARC

Sequenz: HRAELSPSTE NSSQLDFED

Spezifität: This antibody is expected to recognize both reported isoforms (NP_001116423.1, NP_002027.2).

Kreuzreaktivität: Human

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Güteklasse: Verified

Immunogen: Peptide with sequence HRAELSPSTENSSQLDFED-C, from the N Terminus of the protein sequence according to NP_001116423.1, NP_002027.2.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: In paraffin embedded Human Kidney stains epithelial cells of renal tubules and endothelial cells of vessels. The granular staining is compatible with the endoplasmic reticulum association of the protein. Recommended concentration: 3

Western Blot: Approx 37 kDa band observed in Human Liver lysates (calculated MW of 35.6 kDa according to NP_002027.2). Recommended concentration: 0.3-1 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:16000.

Kommentare:

Flow cytometry: Flow cytometric analysis of HEK293 cells. Recommended concentration: 10ug/ml. A customer has reported successful staining of HEK293S-TetR transfected with DARC receptor at a dilution of 1/1000.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu DARC

Target: DARC (Duffy Blood Group, Chemokine Receptor (DARC))

Andere Bezeichnung: ACKR1 (DARC Produkte)

Hintergrund: FY, DARC, HGNC:4035, Duffy blood group, CCBP1, GPD, CTA-134P22.3, CD234, FY, OTTHUMP00000060081, Duffy blood group, chemokine receptor, Duffy blood group antigen, Glycoprotein D, Dfy, GpFy, WBCQ1, Fy glycoprotein, OTTHUMP00000035293, OTTHUMP00000035294

Gen-ID: 2532

NCBI Accession: NP_001116423, NP_002027