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IFT88 Antikörper (C-Term)

Verified IFT88 Reaktivität: Human, Maus WB, ELISA, IF, FACS Wirt: Ziege Polyclonal unconjugated
Produktnummer ABIN185555
  • Target Alle IFT88 Antikörper anzeigen
    IFT88 (Intraflagellar Transport 88 Homolog (IFT88))
    Bindungsspezifität
    • 9
    • 8
    • 7
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    C-Term
    Reaktivität
    • 29
    • 17
    • 7
    • 5
    • 4
    • 4
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Human, Maus
    Wirt
    • 27
    • 2
    Ziege
    Klonalität
    • 28
    • 1
    Polyklonal
    Konjugat
    • 16
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    Dieser IFT88 Antikörper ist unkonjugiert
    Applikation
    • 29
    • 21
    • 21
    • 17
    • 8
    • 3
    • 3
    • 1
    Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)
    Verwendungszweck
    IFT88 / Polaris
    Sequenz
    KKRIDEDDFA DEE
    Spezifität
    This antibody is expected to recognise many reported isoforms.
    Kreuzreaktivität
    Hund, Human, Maus, Ratte
    Aufreinigung
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Güteklasse
    Verified
    Immunogen
    Peptide with sequence C-KKRIDEDDFADEE, from the C Terminus of the protein sequence according to NP_783195.2, NP_006522.2.
    Isotyp
    IgG
  • Applikationshinweise

    Western Blot: Approx 90 kDa band observed in lysates of cell line A549 and in Mouse Testis lysates, and in preliminary testing of Rat Testes lysate (calculated MW of 90.4 kDa according to Hunab NP_001340498.1, and 93.1 kDa according to Mouse NP_033402.2 93.

    Peptide ELISA: antibody detection limit dilution 1:128000.

    Kommentare

    Immunofluorescence: Strong expression of the protein seen in the nucleus and less staining in the cytoplasm of HepG2 cells. Recommended concentration: 10μg/ml.

    Flow Cytometry: Flow cytometric analysis of HepG2 cells.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handhabung
    Minimize freezing and thawing.
    Lagerung
    -20 °C
    Informationen zur Lagerung
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Miyoshi, Kasahara, Miyazaki, Shimizu, Taniguchi, Matsuzaki, Tohyama, Asanuma: "Pericentrin, a centrosomal protein related to microcephalic primordial dwarfism, is required for olfactory cilia assembly in mice." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 23, Issue 10, pp. 3289-97, (2009) (PubMed).

    Jiang, Chiou, Wang, Chien, Ho, Tsai, Lin, Tsai, Li: "Essential role of nephrocystin in photoreceptor intraflagellar transport in mouse." in: Human molecular genetics, Vol. 18, Issue 9, pp. 1566-77, (2009) (PubMed).

  • Target
    IFT88 (Intraflagellar Transport 88 Homolog (IFT88))
    Andere Bezeichnung
    IFT88 (IFT88 Produkte)
    Synonyme
    146590_s_at antikoerper, CG12548 antikoerper, CG14465 antikoerper, CG15190 antikoerper, CG4137 antikoerper, Dmel\\CG12548 antikoerper, IFT88 antikoerper, NOMPB antikoerper, OSM-5 antikoerper, Tg737 antikoerper, nomp antikoerper, osm-5 antikoerper, AW552028 antikoerper, Tg737Rpw antikoerper, TgN737Rpw antikoerper, Ttc10 antikoerper, flexo antikoerper, fxo antikoerper, orpk antikoerper, polaris antikoerper, D13S1056E antikoerper, DAF19 antikoerper, TG737 antikoerper, TTC10 antikoerper, hTg737 antikoerper, intraflagellar transport 88 antikoerper, intraflagellar transport 88 homolog antikoerper, intraflagellar transport protein 88 homolog antikoerper, no mechanoreceptor potential B antikoerper, IFT88 antikoerper, ift88 antikoerper, LOC100518926 antikoerper, LOC100640298 antikoerper, LOC100646898 antikoerper, nompB antikoerper, Ift88 antikoerper
    Hintergrund
    IFT88, polaris, TTC10, tetratricopeptide repeat domain 10, HGNC:20606, D13S1056E, DAF19, IFT88, MGC26259, RP11-172H24.2, TG737, hTg737, TPR repeat protein 10, Probe hTg737 (polycystic kidney disease, autosomal recessive), Tg737 protein, intraflagellar tra
    Gen-ID
    8100, 21821, 305918
    NCBI Accession
    NP_783195, NP_006522, NP_001340507, NP_001340498
    Pathways
    Hedgehog Signalweg, Sensory Perception of Sound
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