IFT88 Antikörper (C-Term)

Produktnummer ABIN185555

Produktdetails anti-IFT88 Antikörper

Target: IFT88 (Intraflagellar Transport 88 Homolog (IFT88))

Bindungsspezifität: C-Term

Reaktivität: Human, Maus

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser IFT88 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Verwendungszweck: IFT88 / Polaris

Sequenz: KKRIDEDDFA DEE

Spezifität: This antibody is expected to recognise many reported isoforms.

Kreuzreaktivität: Hund, Human, Maus, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Güteklasse: Verified

Immunogen: Peptide with sequence C-KKRIDEDDFADEE, from the C Terminus of the protein sequence according to NP_783195.2, NP_006522.2.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Western Blot: Approx 90 kDa band observed in lysates of cell line A549 and in Mouse Testis lysates, and in preliminary testing of Rat Testes lysate (calculated MW of 90.4 kDa according to Hunab NP_001340498.1, and 93.1 kDa according to Mouse NP_033402.2 93.

Peptide ELISA: antibody detection limit dilution 1:128000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the nucleus and less staining in the cytoplasm of HepG2 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HepG2 cells.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Referenzen für anti-IFT88 Antikörper (ABIN185555)

Miyoshi, Kasahara, Miyazaki, Shimizu, Taniguchi, Matsuzaki, Tohyama, Asanuma: "Pericentrin, a centrosomal protein related to microcephalic primordial dwarfism, is required for olfactory cilia assembly in mice." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 23, Issue 10, pp. 3289-97, (2009) (PubMed).

Jiang, Chiou, Wang, Chien, Ho, Tsai, Lin, Tsai, Li: "Essential role of nephrocystin in photoreceptor intraflagellar transport in mouse." in: Human molecular genetics, Vol. 18, Issue 9, pp. 1566-77, (2009) (PubMed).

Details zu IFT88

Target: IFT88 (Intraflagellar Transport 88 Homolog (IFT88))

Andere Bezeichnung: IFT88 (IFT88 Produkte)

Synonyme: 146590_s_at antikoerper, CG12548 antikoerper, CG14465 antikoerper, CG15190 antikoerper, CG4137 antikoerper, Dmel\\CG12548 antikoerper, IFT88 antikoerper, NOMPB antikoerper, OSM-5 antikoerper, Tg737 antikoerper, nomp antikoerper, osm-5 antikoerper, AW552028 antikoerper, Tg737Rpw antikoerper, TgN737Rpw antikoerper, Ttc10 antikoerper, flexo antikoerper, fxo antikoerper, orpk antikoerper, polaris antikoerper, D13S1056E antikoerper, DAF19 antikoerper, TG737 antikoerper, TTC10 antikoerper, hTg737 antikoerper, intraflagellar transport 88 antikoerper, intraflagellar transport 88 homolog antikoerper, intraflagellar transport protein 88 homolog antikoerper, no mechanoreceptor potential B antikoerper, IFT88 antikoerper, ift88 antikoerper, LOC100518926 antikoerper, LOC100640298 antikoerper, LOC100646898 antikoerper, nompB antikoerper, Ift88 antikoerper

Hintergrund: IFT88, polaris, TTC10, tetratricopeptide repeat domain 10, HGNC:20606, D13S1056E, DAF19, IFT88, MGC26259, RP11-172H24.2, TG737, hTg737, TPR repeat protein 10, Probe hTg737 (polycystic kidney disease, autosomal recessive), Tg737 protein, intraflagellar tra

Gen-ID: 8100, 21821, 305918

NCBI Accession: NP_783195, NP_006522, NP_001340507, NP_001340498

Pathways: Hedgehog Signalweg, Sensory Perception of Sound