GLI2 Antikörper (Internal Region)

Produktnummer ABIN185585

Produktdetails anti-GLI2 Antikörper

Target: GLI2 (GLI Family Zinc Finger 2 (GLI2))

Bindungsspezifität: Internal Region

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser GLI2 Antikörper ist unkonjugiert

Applikation: ELISA, Immunohistochemistry (IHC)

Verwendungszweck: GLI2

Sequenz: DLKEDLDRDD CKQE

Spezifität: This antibody is expected to recognise all four reported isoforms (NP_084655.1, NP_084656.1, NP_084657.1 and NP_005261.1)

Homologie: Human, Mouse

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Güteklasse: Verified

Immunogen: Peptide with sequence DLKEDLDRDDCKQE, from the internal region of the protein sequence according to NP_005261.2.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

DS WB Results: Preliminary experiments gave an approx 55 kDa band in Human Bone Marrow and Ovary lysates after 1 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 168 kDa according to NP_005261.2. The 55 kDa band was successfully blocked by incubation with the immunizing peptide.

DS IHC Results: In paraffin embedded Human Lymph Node shows nuclear staining in macrophages. Recommended concentration: 4-6 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:2000.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu GLI2

Target: GLI2 (GLI Family Zinc Finger 2 (GLI2))

Andere Bezeichnung: GLI2 (GLI2 Produkte)

Synonyme: HPE9 antikoerper, THP1 antikoerper, THP2 antikoerper, GLI2/GLI4 antikoerper, GLI3 antikoerper, AW546128 antikoerper, GLI family zinc finger 2 antikoerper, GLI-Kruppel family member GLI2 antikoerper, GLI2 antikoerper, Gli2 antikoerper

Hintergrund: GLI2, GLI-Kruppel family member GLI2 , THP2, oncogene GLI2, tax helper protein 2, tax-responsive element-2 holding protein, tax-responsive element-25-bp sequence binding protein, zinc finger protein GLI2

Gen-ID: 2736

NCBI Accession: NP_005261

Pathways: Hedgehog Signalweg, Dopaminergic Neurogenesis