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Hsc70 Antikörper (Internal Region)

Name: Hsc70 Antikörper (Internal Region)
Brand: antibodies-online
SKU: ABIN185659
Price: 792.57 EUR
Availability: InStock

Verified HSPA8 Reaktivität: Human, Maus WB, IHC, ELISA, IF Wirt: Ziege Polyclonal unconjugated

ABIN185659 (0. (Hsc70 Antikörper (Internal Region))

ABIN185659 (2µg/ml) staining of A431 cell lysate (35µg protein in RIPA buffer). (Hsc70 Antikörper (Internal Region))

ABIN185659 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0. (Hsc70 Antikörper (Internal Region))

3 Abbildungen

(1 reference)

Produktnummer ABIN185659

Datenblatt (PDF)

Target Alle Hsc70 (HSPA8) Antikörper anzeigen

Hsc70 (HSPA8) (Heat Shock 70kDa Protein 8 (HSPA8))
Bindungsspezifität
14

7

6

4

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
Internal Region
Reaktivität
83

48

43

21

17

14

13

11

11

11

10

10

5

5

5

4

4

3

3

3

2

1

1

1

1

1

1

1
Human, Maus
Wirt
72

28

7

1
Ziege
Passende Sekundärantikörper
Klonalität
68

39
Polyklonal
Konjugat
60

19

11

3

3

1

1

1

1

1

1

1

1

1

1

1

1
Dieser Hsc70 Antikörper ist unkonjugiert
Applikation
94

50

40

30

29

28

16

14

11

11

11

5

4

2

2

2

2
Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Verwendungszweck

HSPA8 (Isoform 1)

Sequenz

EKLQGKINDE DKQK

Spezifität

This antibody is expected to recognise isoform 1 (NP_006588.1) only.

Kreuzreaktivität

Hund, Human, Maus, Ratte

Aufreinigung

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Güteklasse

Verified

Immunogen

Peptide with sequence C-EKLQGKINDEDKQK, from the internal region of the protein sequence according to NP_006588.1.

Isotyp

IgG

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Applikationshinweise

Immunohistochemistry: Paraffin embedded Human Prostate. Recommended concentration: 5 μg/mL.
Western Blot: Approx 75 kDa band observed in lysates of cell lines HepG2 and NIH3T3 (calculated MW of 70.9 kDa according to Human NP_006588.1 and Mouse NP_112442.2). This molecular weight is routinely observed by other sources. Recommended concentration:
Peptide ELISA: antibody detection limit dilution 1:4000.

Kommentare

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa cells. Recommended concentration: 10μg/ml

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Liquid

Konzentration

0.5 mg/mL

Buffer

Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel

Sodium azide

Vorsichtsmaßnahmen

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung

Minimize freezing and thawing.

Lagerung

-20 °C

Informationen zur Lagerung

Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
Johnson, Kaplitt: "Novel mitochondrial substrates of omi indicate a new regulatory role in neurodegenerative disorders." in: PLoS ONE, Vol. 4, Issue 9, pp. e7100, (2009) (PubMed).
Target

Hsc70 (HSPA8) (Heat Shock 70kDa Protein 8 (HSPA8))

Andere Bezeichnung

HSPA8 (HSPA8 Produkte)

Hintergrund

HSPA8 , heat shock 70 kDa protein 8 , HSC54, HSC70, HSC71, HSP71, HSP73, HSPA10, LAP, MGC131511, MGC29929, NIP71 , LPS-associated protein 1, N-myristoyltransferase inhibitor protein 71, constitutive heat shock protein 70, heat shock 70kD protein 8, heat sh

Gen-ID

3312, 15481, 24468

NCBI Accession

NP_006588