Melanoma gp100 Antikörper (C-Term)

Produktnummer ABIN185660

Produktdetails anti-Melanoma gp100 Antikörper

Target: Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))

Bindungsspezifität: C-Term

Reaktivität: Human, Maus

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Melanoma gp100 Antikörper ist unkonjugiert

Applikation: Immunofluorescence (IF), Western Blotting (WB), ELISA

Verwendungszweck: Silver homologue / Pmel 17 (C-terminus)

Sequenz: CPIGENSPLL SGQQ

Kreuzreaktivität: Human, Maus

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Güteklasse: Verified

Immunogen: Peptide with sequence CPIGENSPLLSGQQ, from the C Terminus of the protein sequence according to NP_008859.1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Western Blot: Approx 70 kDa band observed in Mouse Skin lysates (calculated MW of 66.3 kDa according to Mouse NP_068682.2). Recommended concentration: 1-3 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:16000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the plasma membrane and cytoplasm of A431cells. Recommended concentration: 10μg/ml.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu Melanoma gp100

Target: Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))

Andere Bezeichnung: PMEL (PMEL Produkte)

Synonyme: D12S53E antikoerper, ME20 antikoerper, ME20-M antikoerper, ME20M antikoerper, P1 antikoerper, P100 antikoerper, PMEL17 antikoerper, SI antikoerper, SIL antikoerper, SILV antikoerper, gp100 antikoerper, D10H12S53E antikoerper, D12S53Eh antikoerper, Pmel17 antikoerper, Si antikoerper, Silv antikoerper, gp87 antikoerper, RPE1 antikoerper, MMP115 antikoerper, silverb antikoerper, silver antikoerper, cb397 antikoerper, fdv antikoerper, pmel17 antikoerper, sb:cb397 antikoerper, silva antikoerper, wu:fc11g11 antikoerper, wu:fj24g11 antikoerper, zgc:136622 antikoerper, premelanosome protein antikoerper, premelanosome protein b antikoerper, premelanosome protein a antikoerper, PMEL antikoerper, Pmel antikoerper, pmelb antikoerper, pmela antikoerper

Hintergrund: Silver homolog (mouse) , PMEL17, SILV , D12S53E, ME20, SI, SIL, gp100 , Melanocyte protein mel 17, Pmel 17, Silver, mouse, homolog of, silver (mouse homolog) like, silver (mouse homolog)-like, silver homolog

Gen-ID: 6490, 20431, 362818

NCBI Accession: NP_008859