Melanoma gp100 Antikörper (C-Term)
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- Target Alle Melanoma gp100 (PMEL) Antikörper anzeigen
- Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))
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Bindungsspezifität
- C-Term
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Reaktivität
- Human, Maus
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Wirt
- Ziege
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Klonalität
- Polyklonal
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Konjugat
- Dieser Melanoma gp100 Antikörper ist unkonjugiert
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Applikation
- Immunofluorescence (IF), Western Blotting (WB), ELISA
- Verwendungszweck
- Silver homologue / Pmel 17 (C-terminus)
- Sequenz
- CPIGENSPLL SGQQ
- Kreuzreaktivität
- Human, Maus
- Aufreinigung
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Güteklasse
- Verified
- Immunogen
- Peptide with sequence CPIGENSPLLSGQQ, from the C Terminus of the protein sequence according to NP_008859.1.
- Isotyp
- IgG
- Top Product
- Discover our top product PMEL Primärantikörper
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- Applikationshinweise
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Western Blot: Approx 70 kDa band observed in Mouse Skin lysates (calculated MW of 66.3 kDa according to Mouse NP_068682.2). Recommended concentration: 1-3 μg/mL. Primary incubation was 1 hour.
Peptide ELISA: antibody detection limit dilution 1:16000.
- Kommentare
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Immunofluorescence: Strong expression of the protein seen in the plasma membrane and cytoplasm of A431cells. Recommended concentration: 10μg/ml.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- 0.5 mg/mL
- Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handhabung
- Minimize freezing and thawing.
- Lagerung
- -20 °C
- Informationen zur Lagerung
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- Target
- Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))
- Andere Bezeichnung
- PMEL (PMEL Produkte)
- Hintergrund
- Silver homolog (mouse) , PMEL17, SILV , D12S53E, ME20, SI, SIL, gp100 , Melanocyte protein mel 17, Pmel 17, Silver, mouse, homolog of, silver (mouse homolog) like, silver (mouse homolog)-like, silver homolog
- Gen-ID
- 6490, 20431, 362818
- NCBI Accession
- NP_008859
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