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CA12 Antikörper (AA 161-318)

CA12 Reaktivität: Maus WB, IHC, IP, ICC Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN1858202
  • Target Alle CA12 Antikörper anzeigen
    CA12 (Carbonic Anhydrase 12 (CA12))
    Bindungsspezifität
    • 15
    • 8
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 161-318
    Reaktivität
    • 48
    • 8
    • 7
    Maus
    Wirt
    • 37
    • 10
    • 2
    Kaninchen
    Klonalität
    • 40
    • 9
    Polyklonal
    Konjugat
    • 26
    • 6
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser CA12 Antikörper ist unkonjugiert
    Applikation
    • 30
    • 22
    • 13
    • 13
    • 13
    • 5
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)
    Verwendungszweck
    Polyclonal Antibody to Carbonic Anhydrase XII (CA12)
    Spezifität
    The antibody is a rabbit polyclonal antibody raised against CA12. It has been selected for its ability to recognize CA12 in immunohistochemical staining and western blotting.
    Kreuzreaktivität
    Human, Ratte
    Aufreinigung
    Antigen-specific affinity chromatography followed by Protein A affinity chromatography
    Immunogen
    Recombinant Carbonic Anhydrase XII (CA12)
    Isotyp
    IgG
    Top Product
    Discover our top product CA12 Primärantikörper
  • Applikationshinweise

    Western blotting: 0.5-2 μg/mL

    1:500-2000 Immunohistochemistry: 5-20 μg/mL

    1:50-200 Immunocytochemistry: 5-20 μg/mL

    1:50-200 Optimal working dilutions must be determined by end user.

    Kommentare

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Validierung #100909 (Immunohistochemistry)
    'Independent Validation' Siegel
    by
    School of Clinical Sciences, University of Bristol
    No.
    #100909
    Datum
    21.02.2017
    Antigen
    CA12
    Chargennummer
    A20170109527
    Validierte Anwendung
    Immunohistochemistry
    Positivkontrolle
    mouse kidney, mouse ciliary body
    Negativkontrolle
    secondary antibody only
    Bewertung
    'Independent Validation' Siegel
    Validierungsbilder
    Protokoll
    Primärantikörper
    ABIN3204699
    Sekundärantikörper
    goat anti-rabbit IgG Alexa Fluor 488 antibody (Life Technology, A-11006, lot 1348202)
    Full Protocol
    • Cryo-section:
      • Wash mouse eye and kidney tissue in PBS.
      • Cryo-protection. Suspend tissue in Optimum Cutting Temperature (OCT) embedding media (Thermo Scientific, Lot 03801137).
      • Gently freeze suspended tissue in liquid nitrogen for 5min.
      • Sections performed on a cryostat (LEICA, CM3050S) set to 12µm.
      • Fix sections in 4% paraformaldehyde (PFA) for 5min at room temperature.
      • Rinse sections in PBS 3 times for 5min.
      • Block sections with 5% normal goat serum for 1h at RT.
    • Immunostaining:
      • Incubate sections with primary rabbit-anti CA12 antibody (antibodies-online, ABIN3204699, lot A20170109527) diluted 1:100 in PBSA, overnight at 4°C. Include a negative control without primary antibody and a kidney positive control.
      • Rinse sections with PBS 3 times for 5min. Keep negative control in a separate jar.
      • Incubate sections with secondary goat anti-rabbit IgG Alexa Fluor 488 antibody conjugate (Life Technology, A-11006, lot 1348202) diluted 1:500 in 1% Bovine Serum Albumin in PBS (PBSA) buffer for 2h at RT.
      • Counterstain with DAPI in the mounting medium step.
    • Wash and mount:
      • Rinse sections in PBS 3 times for 5min.
      • Mount sections in Vectorshield mounting medium with DAPI (Vector laboratories, H-1200, lot ZC0814).
    • Microscopy:
      • Image acquisition on an EVOS FL Color fluorescence and transmitted light microscope (Life technologies, Cat no. AMEFC4300) at 20x magnification.
    Anmerkungen
  • Format
    Liquid
    Konzentration
    Lot specific
    Buffer
    0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.
    Konservierungsmittel
    ProClin
    Vorsichtsmaßnahmen
    WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
    Handhabung
    Avoid repeated freeze-thaw cycles.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
    Haltbarkeit
    24 months
  • Target
    CA12 (Carbonic Anhydrase 12 (CA12))
    Andere Bezeichnung
    Carbonic Anhydrase XII (CA12 Produkte)
    Synonyme
    CAXII antikoerper, HsT18816 antikoerper, CAR12 antikoerper, 2310047E01Rik antikoerper, AI314958 antikoerper, Ca12 antikoerper, ca12 antikoerper, carbonic anhydrase 12 antikoerper, carbonic anhydrase XII L homeolog antikoerper, CA12 antikoerper, Car12 antikoerper, ca12.L antikoerper
    Hintergrund
    CA-XII, CAXII, Carbonate dehydratase XII, Tumor antigen HOM-RCC-3.1.3
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