Histone H4 Antikörper (AA 2-103)

Produktnummer ABIN1859164

Produktdetails anti-Histone H4 Antikörper

Target: Histone H4

Bindungsspezifität: AA 2-103

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Huhn, Schwein, Hund, Meerschweinchen, Pferd, Cat, Rhesusaffen

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Histone H4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Verwendungszweck: Polyclonal Antibody to Histone H4 (H4)

Spezifität: The antibody is a rabbit polyclonal antibody raised against H4. It has been selected for its ability to recognize H4 in immunohistochemical staining and western blotting.

Kreuzreaktivität: Cat, Huhn, Rind (Kuh), Hund, Meerschweinchen, Pferd, Human, Maus, Schwein, Kaninchen, Ratte, Rhesusaffen

Aufreinigung: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Histone H4 (H4) corresdonding to Ser2~Gly103 with N-terminal His and GST Tag

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Western blotting: 0.5-2 μg/mL

1:100-400 Immunohistochemistry: 5-20 μg/mL

1:10-40 Immunocytochemistry: 5-20 μg/mL

1:10-40 Optimal working dilutions must be determined by end user.

Kommentare:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Haltbarkeit: 24 months

Details zu Histone H4

Target: Histone H4

Abstract: Histone H4 Produkte