Dieser Triiodothyronine T3 Antikörper ist konjugiert mit Biotin
Applikation
Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
Spezifität
The antibody is a rabbit polyclonal antibody raised against T3. It has been selected for its ability to recognize T3 in immunohistochemical staining and western blotting.
Aufreinigung
Protein A + Protein G affinity chromatography
Immunogen
The antibody is arabbit polyclonal antibody raised against T3 conjugated to biotin.
T3
Reaktivität: Human
RIA
Wirt: Kaninchen
Polyclonal
unconjugated
Applikationshinweise
Western blotting: 0.2-2µg/mL;1:250-2500 Immunohistochemistry: 5-20µg/mL;1:25-100 Immunocytochemistry: 5-20µg/mL;1:25-100 Optimal working dilutions must be determined by end user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
Lot specific
Buffer
Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handhabung
Avoid repeated freeze/thaw cycles
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.