Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))
Spezifität
M5I-1 reacts with an internal epitope of MRP5 (ABCC5), a 190-200 kDa transmembrane protein that is closely related to the multi-drug resistance protein MRP. M5I-1 does not cross-react with the human MDR1, MRP1, MRP2, or MRP3 gene products. Species Reactivity: Human. Others not tested.
Sterilität
0.22 μm filtered
Immunogen
A bacterial fusion protein containing amino acids 82-168 of human MRP5.Lymph node cells from an immunized rat (Wistar) fused with mouse myeloma (SP2/0) cells.
ABCC5
Reaktivität: Human
Wirt: Kaninchen
Polyclonal
FITC
Applikationshinweise
Flow cytometry: Optimal conditions still to be determined. Immunocytochemistry: Use at a 1:20-1:50 dilution on acetone-fixed cytospin preparations. Immunohistochemistry: Use at a 1:20 dilution on acetone-fixed frozen sections, followed by incubation with rabbit anti-rat IgG and a monoclonal rat APAAP complex. Alternatively, after incubation with primary antibody and washing, slides can be incubated with biotinylated rabbit anti-rat IgG and streptavidin conjugated to horseradish peroxidase. Western blot: Use at a 1:20-1:50 dilution (with anti-rat-HRP). The optimal dilution for a specific application should be determined by the researcher.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Buffer
1 mL (>200 tests) or 0.5 mL (>100 tests) monoclonal antibody at 250 µg IgG/mL in serum-free culture supernatant with protein stabilizer and 0.1% sodium azide. Filtered through a 0.22 µm filter. Culture medium: IMDM, supplemented with Nutridoma-NS/SP. Th
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
4 °C
Target
ABCC5
(ATP-Binding Cassette, Sub-Family C (CFTR/MRP), Member 5 (ABCC5))