This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogen
This IKKB Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S177 of human IKKB.
IKBKB
Reaktivität: Human
ELISA, WB, IHC, IF
Wirt: Kaninchen
Monoclonal
3E1
unconjugated
Recombinant Antibody
Applikationshinweise
DB: 1:500
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Buffer
Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
4 °C,-20 °C
Haltbarkeit
6 months
Yatherajam, Banerjee, McCorkell, Solt, Hanson, Madge, Kang, Worley, Orange, May: "Cutting edge: association with I kappa B kinase beta regulates the subcellular localization of Homer3." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 185, Issue 5, pp. 2665-9, (2010) (PubMed).
Kenneth, Mudie, Rocha: "IKK and NF-kappaB-mediated regulation of Claspin impacts on ATR checkpoint function." in: The EMBO journal, Vol. 29, Issue 17, pp. 2966-78, (2010) (PubMed).
Zhao, Li, Chen: "CC2D1A, a DM14 and C2 domain protein, activates NF-kappaB through the canonical pathway." in: The Journal of biological chemistry, Vol. 285, Issue 32, pp. 24372-80, (2010) (PubMed).
Niida, Tanaka, Kamitani: "Downregulation of active IKK beta by Ro52-mediated autophagy." in: Molecular immunology, Vol. 47, Issue 14, pp. 2378-87, (2010) (PubMed).
Schuurhof, Bont, Siezen, Hodemaekers, van Houwelingen, Kimman, Hoebee, Kimpen, Janssen: "Interleukin-9 polymorphism in infants with respiratory syncytial virus infection: an opposite effect in boys and girls." in: Pediatric pulmonology, Vol. 45, Issue 6, pp. 608-13, (2010) (PubMed).
Target
IKBKB
(Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase beta (IKBKB))
NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA (MIM 164014), or RELB (MIM 604758) to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008, or NFKBIB, MIM 604495), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T, R is an A or G purine, and Y is a C or T pyrimidine).