Topoisomerase I Antikörper (N-Term)

Produktnummer ABIN1882055

Produktdetails anti-Topoisomerase I Antikörper

Target: Topoisomerase I (TOP1) (Topoisomerase (DNA) I (TOP1))

Bindungsspezifität: N-Term

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Topoisomerase I Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS)

Aufreinigung: This antibody is purified through a protein G column, followed by dialysis against PBS.

Immunogen: This TOP1 antibody is generated from a mouse immunized with a recombinant protein from the N-terminal region of human TOP1.

Klon: 1291CT875-142-166

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: WB: 1:1000. FC: 1:25

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Purified monoclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Haltbarkeit: 6 months

Referenzen für anti-Topoisomerase I Antikörper (ABIN1882055)

Deloukas, Matthews, Ashurst, Burton, Gilbert, Jones, Stavrides, Almeida, Babbage, Bagguley, Bailey, Barlow, Bates, Beard, Beare, Beasley, Bird, Blakey, Bridgeman, Brown, Buck, Burrill, Butler, Carder et al.: "The DNA sequence and comparative analysis of human chromosome 20. ..." in: Nature, Vol. 414, Issue 6866, pp. 865-71, (2002) (PubMed).

Kunze, Yang, Dölberg, Sundarp, Knippers, Richter: "Structure of the human type I DNA topoisomerase gene." in: The Journal of biological chemistry, Vol. 266, Issue 15, pp. 9610-6, (1991) (PubMed).

Details zu Topoisomerase I

Target: Topoisomerase I (TOP1) (Topoisomerase (DNA) I (TOP1))

Andere Bezeichnung: TOP1 (TOP1 Produkte)

Synonyme: CG6146 antikoerper, Dmel\\CG6146 antikoerper, TOP1 antikoerper, TopI antikoerper, Topo I antikoerper, TopoI antikoerper, dTopoI antikoerper, l(1)G0134 antikoerper, l(1)G0201 antikoerper, l(1)G0229 antikoerper, l(1)G0278 antikoerper, l(1)top1 antikoerper, top1 antikoerper, topo I antikoerper, topoI antikoerper, DNA topoisomerase 1 beta antikoerper, DNA topoisomerase I alpha antikoerper, MGO1 antikoerper, MGOUN 1 antikoerper, MTE17.1 antikoerper, MTE17_1 antikoerper, TOP1BETA antikoerper, TOPOISOMERASE 1 antikoerper, TOPI antikoerper, AI467334 antikoerper, D130064I21Rik antikoerper, Top-1 antikoerper, Ab2-086 antikoerper, topi antikoerper, Topoisomerase 1 antikoerper, DNA topoisomerase I antikoerper, DNA topoisomerase I alpha antikoerper, DNA topoisomerase 1 antikoerper, topoisomerase (DNA) I antikoerper, DNA topoisomerase I, gene 1 L homeolog antikoerper, Top1 antikoerper, top1 antikoerper, topA antikoerper, ZMO1193 antikoerper, MRAD2831_RS63095 antikoerper, Lferr_0016 antikoerper, PC1_2015 antikoerper, Za10_0136 antikoerper, Gbro_0575 antikoerper, Dd586_2108 antikoerper, Kvar_3044 antikoerper, Alvin_2069 antikoerper, Dacet_0849 antikoerper, Mrub_1615 antikoerper, Arnit_2962 antikoerper, Ndas_4953 antikoerper, Trad_2377 antikoerper, Acear_1653 antikoerper, Mahau_0844 antikoerper, Mesop_4109 antikoerper, Thein_1586 antikoerper, Theth_0241 antikoerper, TOP1ALPHA antikoerper, top-1 antikoerper, TOP1 antikoerper, top1.1.L antikoerper

Substanzklasse: Viral Protein

Hintergrund: Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells.

Molekulargewicht: 90726

UniProt: P11387

Pathways: Caspase Kaskade in der Apoptose, Stem Cell Maintenance