Topoisomerase I Antikörper (N-Term)

Produktnummer ABIN1882055

Produktdetails anti-Topoisomerase I Antikörper

Target: Topoisomerase I (TOP1) (Topoisomerase (DNA) I (TOP1))

Bindungsspezifität: N-Term

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Topoisomerase I Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS)

Aufreinigung: This antibody is purified through a protein G column, followed by dialysis against PBS.

Immunogen: This TOP1 antibody is generated from a mouse immunized with a recombinant protein from the N-terminal region of human TOP1.

Klon: 1291CT875-142-166

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: WB: 1:1000. FC: 1:25

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Purified monoclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Haltbarkeit: 6 months

Referenzen für anti-Topoisomerase I Antikörper (ABIN1882055)

Deloukas, Matthews, Ashurst, Burton, Gilbert, Jones, Stavrides, Almeida, Babbage, Bagguley, Bailey, Barlow, Bates, Beard, Beare, Beasley, Bird, Blakey, Bridgeman, Brown, Buck, Burrill, Butler, Carder et al.: "The DNA sequence and comparative analysis of human chromosome 20. ..." in: Nature, Vol. 414, Issue 6866, pp. 865-71, (2002) (PubMed).

Kunze, Yang, Dölberg, Sundarp, Knippers, Richter: "Structure of the human type I DNA topoisomerase gene." in: The Journal of biological chemistry, Vol. 266, Issue 15, pp. 9610-6, (1991) (PubMed).

Details zu Topoisomerase I

Target: Topoisomerase I (TOP1) (Topoisomerase (DNA) I (TOP1))

Andere Bezeichnung: TOP1 (TOP1 Produkte)

Substanzklasse: Viral Protein

Hintergrund: Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells.

Molekulargewicht: 90726

UniProt: P11387

Pathways: Caspase Kaskade in der Apoptose, Stem Cell Maintenance