This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Immunogen
This RENT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 7-37 amino acids from the N-terminal region of human RENT1.
UPF1
Reaktivität: Human
WB, ELISA
Wirt: Maus
Monoclonal
4G3
unconjugated
Applikationshinweise
WB: 1:1000
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Buffer
Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
4 °C,-20 °C
Haltbarkeit
6 months
Kang, Shen, Macdonald: "Two molecular pathways (NMD and ERAD) contribute to a genetic epilepsy associated with the GABA(A) receptor GABRA1 PTC mutation, 975delC, S326fs328X." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 29, Issue 9, pp. 2833-44, (2009) (PubMed).
Ohnishi, Yamashita, Kashima, Schell, Anders, Grimson, Hachiya, Hentze, Anderson, Ohno: "Phosphorylation of hUPF1 induces formation of mRNA surveillance complexes containing hSMG-5 and hSMG-7." in: Molecular cell, Vol. 12, Issue 5, pp. 1187-200, (2003) (PubMed).
Mendell, ap Rhys, Dietz: "Separable roles for rent1/hUpf1 in altered splicing and decay of nonsense transcripts." in: Science (New York, N.Y.), Vol. 298, Issue 5592, pp. 419-22, (2002) (PubMed).
Lykke-Andersen: "Identification of a human decapping complex associated with hUpf proteins in nonsense-mediated decay." in: Molecular and cellular biology, Vol. 22, Issue 23, pp. 8114-21, (2002) (PubMed).
Carastro, Tan, Selg, Jack, So, Downey: "Identification of delta helicase as the bovine homolog of HUPF1: demonstration of an interaction with the third subunit of DNA polymerase delta." in: Nucleic acids research, Vol. 30, Issue 10, pp. 2232-43, (2002) (PubMed).
Target
RENT1/UPF1 (UPF1)
(UPF1 Regulator of Nonsense Transcripts Homolog (UPF1))
RENT1 is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein is located only in the cytoplasm. When translation ends, it interacts with the protein that is a functional homolog of yeast Upf2p to trigger mRNA decapping.