MGMT
Reaktivität: Human
WB, IF
Wirt: Maus
Polyclonal
unconjugated
Applikationshinweise
IF: 1:25. WB: 1:1000. IHC: 1:100. IHC-P: 1:25
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Buffer
Purified monoclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
4 °C,-20 °C
Haltbarkeit
6 months
Edara, Goodtzova, Pegg: "The role of tyrosine-158 in O6-alkylguanine-DNA alkyltransferase activity." in: Carcinogenesis, Vol. 16, Issue 7, pp. 1637-42, (1995) (PubMed).
Tano, Shiota, Collier, Foote, Mitra: "Isolation and structural characterization of a cDNA clone encoding the human DNA repair protein for O6-alkylguanine." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 87, Issue 2, pp. 686-90, (1990) (PubMed).
Rydberg, Spurr, Karran: "cDNA cloning and chromosomal assignment of the human O6-methylguanine-DNA methyltransferase. cDNA expression in Escherichia coli and gene expression in human cells." in: The Journal of biological chemistry, Vol. 265, Issue 16, pp. 9563-9, (1990) (PubMed).
Koike, Maki, Takeya, Hayakawa, Sekiguchi: "Purification, structure, and biochemical properties of human O6-methylguanine-DNA methyltransferase." in: The Journal of biological chemistry, Vol. 265, Issue 25, pp. 14754-62, (1990) (PubMed).
Hayakawa, Koike, Sekiguchi: "Expression and cloning of complementary DNA for a human enzyme that repairs O6-methylguanine in DNA." in: Journal of molecular biology, Vol. 213, Issue 4, pp. 739-47, (1990) (PubMed).
Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.