YY1 Antikörper

Produktnummer ABIN1882292

Produktdetails anti-YY1 Antikörper

Target: YY1 (YY1 Transcription Factor (YY1))

Reaktivität: Human, Ratte, Zebrafisch (Danio rerio), Cynomolgus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser YY1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Aufreinigung: This antibody is purified through a protein G column, followed by dialysis against PBS.

Immunogen: Purified His-tagged YY1 protein was used to produced this monoclonal antibody.

Klon: 1183CT6-5-23-6

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: WB: 1:500-1:1000. WB: 1:1000. IHC-P: 1:25. IHC-P: 1:25. IHC-P: 1:25

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Purified monoclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Haltbarkeit: 6 months

Referenzen für anti-YY1 Antikörper (ABIN1882292)

Fernando, Contreras, Zampini, Rodriguez-Malave, Alberti, Anguiano, Tran, Palanichamy, Gajeton, Ung, Aros, Waters, Casero, Basso, Pigazzi, Rao: "The lncRNA CASC15 regulates SOX4 expression in RUNX1-rearranged acute leukemia." in: Molecular cancer, Vol. 16, Issue 1, pp. 126, (2018) (PubMed).

Burkard, Planyavsky, Kaupe, Breitwieser, Bürckstümmer, Bennett, Superti-Furga, Colinge: "Initial characterization of the human central proteome." in: BMC systems biology, Vol. 5, pp. 17, (2011) (PubMed).

Chen, Cai, Jin, Florens, Swanson, Washburn, Conaway, Conaway: "Subunit organization of the human INO80 chromatin remodeling complex: an evolutionarily conserved core complex catalyzes ATP-dependent nucleosome remodeling." in: The Journal of biological chemistry, Vol. 286, Issue 13, pp. 11283-9, (2011) (PubMed).

Kim, Kim: "YY1's longer DNA-binding motifs." in: Genomics, Vol. 93, Issue 2, pp. 152-8, (2009) (PubMed).

Details zu YY1

Target: YY1 (YY1 Transcription Factor (YY1))

Andere Bezeichnung: YY1 (YY1 Produkte)

Hintergrund: Multifunctional transcription factor that exhibits positive and negative control on a large number of cellular and viral genes by binding to sites overlapping the transcription start site. Binds to the consensus sequence 5'-CCGCCATNTT-3', some genes have been shown to contain a longer binding motif allowing enhanced binding, the initial CG dinucleotide can be methylated greatly reducing the binding affinity. The effect on transcription regulation is depending upon the context in which it binds and diverse mechanisms of action include direct activation or repression, indirect activation or repression via cofactor recruitment, or activation or repression by disruption of binding sites or conformational DNA changes. Its activity is regulated by transcription factors and cytoplasmic proteins that have been shown to abrogate or completely inhibit YY1-mediated activation or repression. For example, it acts as a repressor in absence of adenovirus E1A protein but as an activator in its presence. May play an important role in development and differentiation. Proposed to recruit the PRC2/EED-EZH2 complex to target genes that are transcriptional repressed. Involved in DNA repair. In vitro, binds to DNA recombination intermediate structures (Holliday junctions). Proposed core component of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA replication and probably DNA repair, proposed to target the INO80 complex to YY1-responsive elements.

Molekulargewicht: 44713

NCBI Accession: NP_003394

UniProt: P25490