Nitrogenase Antikörper

Produktnummer ABIN190742

Produktdetails anti-Nitrogenase Antikörper

Target: Nitrogenase (Nifh)

Reaktivität: Rhodopseudomonas palustris

Wirt: Huhn

Klonalität: Polyklonal

Applikation: Western Blotting (WB)

Kreuzreaktivität (Details): No cross-reactivity with: Synechococcus sp. PCC 7942 and Synechocystis

Homologie: Desulfotomaculum reducens (strain MI-1),Clostridium cellobioparum, Frankia sp. (strain EAN1pec), Magnetococcus sp., Methanobacterium thermoautotrophicum, Methanococcus maripaludis, Methylobacterium sp , Rhodopseudomonas palustris TIE-1 strain, alpha,gamma,beta proteobacteria, enterobacteria, low GC gram+, high GC gram +, euryachaeotes, Azotobacter vinelandii (Gram-), Klebsiella pneumonia, cyanobacteria, Enterobacter genera, able to fix atmoshperic nitrogen

Produktmerkmale: Expected / apparent Molecular Weight of the Antigene: 27 / 32.5 kDa

Aufreinigung: affinity purified

Immunogen: KLH-conjugated synthetic peptide derived from known bacterial NifH subunits of bacterial nitrogenase enzymes of the FeMoCo type including Synechoccocus sp. Q2JP78 , Trichodesmium theibautii, Anabaena sp. P33178 and Nostoc sp. Q51296

Anwendungsinformationen

Applikationshinweise: Recommended Dilution 1 : 2 000 (WB).

Kommentare:

This antibody is not suitable for immunolocalization studies on bacterial cultures.An enzyme involved in chlorophyll synthesis, present in all cyanobacteria (fixing and non-nitrogen fixing) is a member of the NifH family/superfamily. Agrisera anti-NifH antibody will not show a strong reactivity to this target.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.36 µg/µL

Buffer: PBS pH 8.0+ 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.
Make aliquots to avoid repreated freeze-thaw cycles and working with a stock.

Lagerung: 4 °C

Referenzen für anti-Nitrogenase Antikörper (ABIN190742)

Halimatul, Ehira, Awai: "Fatty alcohols can complement functions of heterocyst specific glycolipids in Anabaena sp. PCC 7120." in: Biochemical and biophysical research communications, Vol. 450, Issue 1, pp. 178-83, (2014) (PubMed).

Levitan, Brown, Sudhaus, Campbell, LaRoche, Berman-Frank: "Regulation of nitrogen metabolism in the marine diazotroph Trichodesmium IMS101 under varying temperatures and atmospheric CO2 concentrations." in: Environmental microbiology, Vol. 12, Issue 7, pp. 1899-912, (2010) (PubMed).

Details zu Nitrogenase

Target: Nitrogenase (Nifh)

Abstract: Nifh Produkte

Hintergrund: Nitrogenase is involved in biological fixation of atmospheric nitrogen to ammonia. Alternative protein names: nitrogenase component II, nitrogenase Fe protein, nitrogenase reductase, FeMoCo-nitrogenase.

Molekulargewicht: expected: 27 kDa, apparent: 32.5 kDa

UniProt: P26254, Q2JP78, P33178, Q51296