ASS1 Antikörper (Internal Region)
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- Target Alle ASS1 Antikörper anzeigen
- ASS1 (Argininosuccinate Synthase 1 (ASS1))
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Bindungsspezifität
- Internal Region
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Reaktivität
- Human, Maus, Ratte
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Wirt
- Ziege
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Klonalität
- Polyklonal
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Konjugat
- Dieser ASS1 Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)
- Verwendungszweck
- Argininosuccinate synthetase 1
- Sequenz
- ENPKNQAPPG LYTKTQD
- Spezifität
- The variants represent identical protein (NP_000041.2 and NP_446464.1).
- Kreuzreaktivität
- Rind (Kuh), Hund, Human, Maus, Ratte
- Aufreinigung
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Güteklasse
- Verified
- Immunogen
- C-ENPKNQAPPGLYTKTQD
- Isotyp
- IgG
- Top Product
- Discover our top product ASS1 Primärantikörper
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- Applikationshinweise
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Immunohistochemistry: In paraffin embedded Human Kidney shows textured cytoplasm staining in PCT. Recommended concentration: 2-4 μg/mL. Paraffin embedded Human Liver. Recommended concentration: 2.5 μg/mL.
Western Blot: Approx 45 kDa band observed in lysates of cell lines A431 and NIH3T3 and approx.48 kDa in Human and Rat Kidney and in Mouse Liver lysates (calculated MW of 46.5 kDa according to Human NP_000041.2 and Rat NP_037289.1, and 46.6 kDa according to
Peptide ELISA: antibody detection limit dilution 1:64000.
- Kommentare
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Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.<
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- 0.5 mg/mL
- Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handhabung
- Minimize freezing and thawing.
- Lagerung
- -20 °C
- Informationen zur Lagerung
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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Phosphorylation of argininosuccinate synthase by protein kinase A." in: Biochemical and biophysical research communications, Vol. 377, Issue 4, pp. 1042-6, (2008) (PubMed).
: "
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Phosphorylation of argininosuccinate synthase by protein kinase A." in: Biochemical and biophysical research communications, Vol. 377, Issue 4, pp. 1042-6, (2008) (PubMed).
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- Target
- ASS1 (Argininosuccinate Synthase 1 (ASS1))
- Andere Bezeichnung
- ASS1 (ASS1 Produkte)
- Synonyme
- ASS antikoerper, CTLN1 antikoerper, AA408052 antikoerper, Ass-1 antikoerper, fold antikoerper, ASSA antikoerper, Ass antikoerper, ass antikoerper, zgc:92051 antikoerper, wu:fb95a04 antikoerper, wu:fc01e08 antikoerper, argininosuccinate synthase 1 antikoerper, argininosuccinate synthetase 1 antikoerper, argininosuccinate synthase 1 S homeolog antikoerper, ASS1 antikoerper, Ass1 antikoerper, ass1.S antikoerper, ass1 antikoerper
- Hintergrund
- Argininosuccinate synthetase 1, ASS, ASS1, CTLN1, citrulline--aspartate ligase
- Gen-ID
- 445, 11898, 25698
- NCBI Accession
- NP_000041, NP_446464
- Pathways
- Response to Growth Hormone Stimulus, Cellular Response to Molecule of Bacterial Origin
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