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ASS1 Antikörper (Internal Region)

Verified ASS1 Reaktivität: Human, Maus, Ratte WB, ELISA, IHC, FACS, IF Wirt: Ziege Polyclonal unconjugated
Produktnummer ABIN190834
  • Target Alle ASS1 Antikörper anzeigen
    ASS1 (Argininosuccinate Synthase 1 (ASS1))
    Bindungsspezifität
    • 12
    • 8
    • 7
    • 6
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Internal Region
    Reaktivität
    • 59
    • 17
    • 12
    • 5
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    Human, Maus, Ratte
    Wirt
    • 49
    • 8
    • 2
    Ziege
    Klonalität
    • 50
    • 9
    Polyklonal
    Konjugat
    • 38
    • 4
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser ASS1 Antikörper ist unkonjugiert
    Applikation
    • 46
    • 32
    • 27
    • 18
    • 11
    • 8
    • 5
    • 3
    • 3
    • 2
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)
    Verwendungszweck
    Argininosuccinate synthetase 1
    Sequenz
    ENPKNQAPPG LYTKTQD
    Spezifität
    The variants represent identical protein (NP_000041.2 and NP_446464.1).
    Kreuzreaktivität
    Rind (Kuh), Hund, Human, Maus, Ratte
    Aufreinigung
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Güteklasse
    Verified
    Immunogen
    C-ENPKNQAPPGLYTKTQD
    Isotyp
    IgG
    Top Product
    Discover our top product ASS1 Primärantikörper
  • Applikationshinweise

    Immunohistochemistry: In paraffin embedded Human Kidney shows textured cytoplasm staining in PCT. Recommended concentration: 2-4 μg/mL. Paraffin embedded Human Liver. Recommended concentration: 2.5 μg/mL.

    Western Blot: Approx 45 kDa band observed in lysates of cell lines A431 and NIH3T3 and approx.48 kDa in Human and Rat Kidney and in Mouse Liver lysates (calculated MW of 46.5 kDa according to Human NP_000041.2 and Rat NP_037289.1, and 46.6 kDa according to

    Peptide ELISA: antibody detection limit dilution 1:64000.

    Kommentare

    Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa cells. Recommended concentration: 10μg/ml.

    Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.<

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handhabung
    Minimize freezing and thawing.
    Lagerung
    -20 °C
    Informationen zur Lagerung
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Corbin, Pendleton, Solomonson, Eichler: "Phosphorylation of argininosuccinate synthase by protein kinase A." in: Biochemical and biophysical research communications, Vol. 377, Issue 4, pp. 1042-6, (2008) (PubMed).

  • Target
    ASS1 (Argininosuccinate Synthase 1 (ASS1))
    Andere Bezeichnung
    ASS1 (ASS1 Produkte)
    Synonyme
    ASS antikoerper, CTLN1 antikoerper, AA408052 antikoerper, Ass-1 antikoerper, fold antikoerper, ASSA antikoerper, Ass antikoerper, ass antikoerper, zgc:92051 antikoerper, wu:fb95a04 antikoerper, wu:fc01e08 antikoerper, argininosuccinate synthase 1 antikoerper, argininosuccinate synthetase 1 antikoerper, argininosuccinate synthase 1 S homeolog antikoerper, ASS1 antikoerper, Ass1 antikoerper, ass1.S antikoerper, ass1 antikoerper
    Hintergrund
    Argininosuccinate synthetase 1, ASS, ASS1, CTLN1, citrulline--aspartate ligase
    Gen-ID
    445, 11898, 25698
    NCBI Accession
    NP_000041, NP_446464
    Pathways
    Response to Growth Hormone Stimulus, Cellular Response to Molecule of Bacterial Origin
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