DGAT1 Antikörper (Internal Region)

Produktnummer ABIN190886

Produktdetails anti-DGAT1 Antikörper

Target: DGAT1 (Diacylglycerol O-Acyltransferase 1 (DGAT1))

Bindungsspezifität: Internal Region

Reaktivität: Human, Maus

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser DGAT1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Verwendungszweck: DGAT1

Sequenz: QNSMKPFKDM DYS

Kreuzreaktivität: Rind (Kuh), Hund, Human, Maus, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Güteklasse: Verified

Immunogen: Peptide with sequence C-QNSMKPFKDMDYS, from the internal region of the protein sequence according to NP_036211.1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: Paraffin embedded Human Colon. Recommended concentration: 3.75 μg/mL.

Western Blot: Approx 55 kDa band observed in Mouse Liver lysates (calculated MW of 56.8 kDa according to NP_034176.1). Recommended concentration: 0.01-0.1.0 μg/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:64000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the endoplasmic reticulum and cytoplasm of A431 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommende

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu DGAT1

Target: DGAT1 (Diacylglycerol O-Acyltransferase 1 (DGAT1))

Andere Bezeichnung: DGAT1 (DGAT1 Produkte)

Hintergrund: DGAT1, diacylglycerol O-acyltransferase homolog 1 (mouse) , ARGP1, DGAT , ACAT related gene product 1, acyl coenzyme A:cholesterol acyltransferase related gene 1, diacylglycerol O-acyltransferase 1, diglyceride acyltransferase

Gen-ID: 8694, 13350, 84497

NCBI Accession: NP_036211

Pathways: Hormone Transport