PIAS2 Antikörper (Internal Region)

Produktnummer ABIN190909

Produktdetails anti-PIAS2 Antikörper

Target: PIAS2 (Protein Inhibitor of Activated STAT, 2 (PIAS2))

Bindungsspezifität: Internal Region

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser PIAS2 Antikörper ist unkonjugiert

Applikation: ELISA, Immunohistochemistry (IHC)

Verwendungszweck: PIAS2

Sequenz: KEAMKVSSQP CTKIE

Spezifität: This antibody is expected to recognise both reported isoforms (NP_775298.1 and NP_004662.2). No cross-reactivity expected with PIAS1, 3 and 4.

Kreuzreaktivität: Hund, Human, Maus, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Güteklasse: Verified

Immunogen: Peptide with sequence KEAMKVSSQPCTKIE, from the internal region of the protein sequence according to NP_775298.1, NP_004662.2.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: In paraffin embedded Human Testis shows nuclear and extra-nuclear staining in spermatids. Recommended concentration, 3-5 μg/mL.

Western Blot: Preliminary experiments gave an approx 90 kDa band in lysates of cell line A431after 0.5 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of

Peptide ELISA: antibody detection limit dilution 1:8000.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu PIAS2

Target: PIAS2 (Protein Inhibitor of Activated STAT, 2 (PIAS2))

Andere Bezeichnung: PIAS2 (PIAS2 Produkte)

Hintergrund: PIAS2, protein inhibitor of activated STAT, 2 , MGC102682, MIZ1, PIASX-ALPHA, PIASX-BETA, SIZ2, miz , Msx-interacting-zinc finger, protein inhibitor of activated STAT X, ARIP3

Gen-ID: 9063, 17344

NCBI Accession: NP_775298, NP_004662

Pathways: JAK-STAT Signalweg, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling