MAPK6 Antikörper (AA 683-694)
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- Target Alle MAPK6 Antikörper anzeigen
- MAPK6 (Mitogen-Activated Protein Kinase 6 (MAPK6))
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Bindungsspezifität
- AA 683-694
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Reaktivität
- Human, Maus, Rind (Kuh), Hund, Pferd, Kaninchen, Schwein, Huhn, Hamster
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Wirt
- Ziege
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Klonalität
- Polyklonal
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Konjugat
- Dieser MAPK6 Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Marke
- IHC-plus™
- Spezifität
- Human MAPK6 / ERK3. Reported variants represent identical protein (NP_056621.4, NP_081694.1).
- Aufreinigung
- Immunoaffinity purified
- Immunogen
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Synthetic peptide C-HSPVGSPLKSIQ from an internal region (near C-terminus) of human MAPK6 / ERK3 (NP_002739.1). Percent identity by BLAST analysis: Hamster, Elephant, Panda, Dog, Bovine, Cat, Horse, Rabbit, Pig, Opossum, Turkey, Zebra finch, Chicken, Platypus, Lizard (100%), Rat, Xenopus (92%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product MAPK6 Primärantikörper
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- Applikationshinweise
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Approved: IHC, IHC-P (10 μg/mL), Peptide-ELISA (1:128000), WB (0.5 - 1 μg/mL)
Usage: Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: In transfected HEK293 transiently expressing Mouse ERK3 / Mapk6 fused to GFP the band with expected size is intensified when co-expressed with MK5 as the stabilizer for ERK3. No bands are observed in transfected HEK293 with empty vector and the same band is observed using anti-GFP antibody. Data obtained from Dr. M. B. Menon, Inst. Biochemistry, Hannover Medical School, Germany. Recommended concentration, 0.5-1 μg/mL. Immunoprecipitation: MK5/ERK3 double knockout Mouse Embryonic Fibroblasts (MEFs) retrovirally transduced with MK5/ERK3 or empty vector (GFP) were lysed from confluent plates and used for IP with 1.5 μg EB11926. Western blots of the IP were labeled with rabbit anti-ERK3 serum (CST) or with mouse anti-MK5 (SC). An approx 100kD of ERK3 is only precipitated from lysates of those KO MEFs that have been rescued by the ERK3/MK5 expression construct as described in as described in PMID: 22508986. Data obtained from Dr. M. B. Menon, Inst. Biochemistry, Hannover Medical School, Germany. - Kommentare
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Target Species of Antibody: Human
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- Lot specific
- Buffer
- Tris-buffered saline, pH 7.3, 0.5 % BSA, 0.02 % sodium azide
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handhabung
- Minimize freezing and thawing.
- Lagerung
- -20 °C
- Informationen zur Lagerung
- Store at -20°C. Avoid freeze-thaw cycles.
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- Target
- MAPK6 (Mitogen-Activated Protein Kinase 6 (MAPK6))
- Andere Bezeichnung
- MAPK6 / ERK3 (MAPK6 Produkte)
- Synonyme
- ERK3 antikoerper, HsT17250 antikoerper, PRKM6 antikoerper, p97MAPK antikoerper, 2610021I23Rik antikoerper, D130053K17Rik antikoerper, Erk3 antikoerper, Mapk4 antikoerper, Mapk63 antikoerper, Prkm4 antikoerper, Prkm6 antikoerper, ATMAPK6 antikoerper, ATMPK6 antikoerper, F18O19.10 antikoerper, MAP KINASE 6 antikoerper, MAP kinase 6 antikoerper, MAPK6 antikoerper, SUSERK3 antikoerper, erk3 antikoerper, p97mapk antikoerper, si:ch211-235f12.1 antikoerper, mitogen-activated protein kinase 6 antikoerper, MAP kinase 6 antikoerper, MAPK6 antikoerper, Mapk6 antikoerper, MPK6 antikoerper, mapk6 antikoerper
- Hintergrund
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Name/Gene ID: MAPK6
Subfamily: MAPK
Family: Protein Kinase
Synonyms: MAPK6, ERK-3, MAP kinase 6, MAPK 6, p97-MAPK, PRKM6, MAP kinase isoform p97, ERK3, HsT17250, p97MAPK - Gen-ID
- 5597
- NCBI Accession
- NP_002739
- Pathways
- MAPK Signalweg, Neurotrophin Signalübertragung, Regulation of Muscle Cell Differentiation, Hepatitis C
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