This antibody recognizes Human Bone Sialoprotein, Recombinant Human, Recombinant Porcine and Recombinant Rat BSP. There was no cross reactivity obtained with Human, Porcine Osteonectin and Osteopontin.
Kreuzreaktivität (Details)
Species reactivity (tested):Human, Porcine, Rat.
Produktmerkmale
Synonyms: Bone sialoprotein II, BSP II, IBSP, BNSP, Cell-binding sialoprotein, Integrin-bindingsialoprotein
IBSP
Reaktivität: Human
WB, IF (cc), IF (p)
Wirt: Kaninchen
Polyclonal
AbBy Fluor® 555
Applikationshinweise
ELISA (3). RIA. Western blot: 1/1000 (1,3). Immunocytochemstry: 1/200 (3). Immunohistochemistry on CryoSections, Resin-Embedded Sections: 1/100 (2,3,5,6). Immunohistochemistry on Paraffin Sections: 1/2000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Rekonstitution
Restore in aqua bidest to 1 mg/mL.
Buffer
50 mM TRIS, pH 7.4
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Store lyophilized at 2-8 °C and reconstituted at -20 °C. Avoid repeated freezing and thawing. Shelf life: One year from despatch.
The protein encoded by the Bone Sialoprotein gene is a major structural protein of the bone matrix. It is synthesized by skeletal-associated cell types, including hypertrophic chondrocytes, osteoblasts, osteocytes, and osteoclasts, and it constitutes approximately 12 % of the noncollagenous proteins in human bone. The only extraskeletal site of its synthesis is the trophoblast. This protein binds to calcium and hydroxyapatite via its acidic amino acid clusters, and mediates cell attachment through an RGD sequence that recognizes the vitronectin receptor.Synonyms: BNSP, BSP II, Bone sialoprotein II, Cell-binding sialoprotein, IBSP, Integrin-binding sialoprotein