CD6 Antikörper (PE)

Produktnummer ABIN192058

Produktdetails anti-CD6 Antikörper (PE)

Target: CD6

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD6 Antikörper ist konjugiert mit PE

Applikation: Flow Cytometry (FACS)

Spezifität: The antibody MEM-98 reacts with an extracellular epitope of CD6, a 100-130 kDa single chain transmembrane glycoprotein expressed on T and B lymphocytes subsets, thymocytes, and acute lymphocytic leukemia cells.

Kreuzreaktivität (Details): Human

Aufreinigung: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: Human CD6 antigen purified by immunoaffinity chromatography from HBP-ALL cells followed by preparative SDS-PAGE of non-boiled non-reduced sample (excised piece of gel corresponding to the 100 kDa zone).

Klon: MEM-98

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.

Kommentare:

The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: No reconstitution is necessary.

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do not freeze.
Avoid prolonged exposure to light.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Referenzen für anti-CD6 Antikörper (PE) (ABIN192058)

Castro, Oliveira, Nunes, Fabre, Barbosa, Peixoto, Brown, Parnes, Bismuth, Moreira, Rocha, Carmo: "Extracellular isoforms of CD6 generated by alternative splicing regulate targeting of CD6 to the immunological synapse." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 178, Issue 7, pp. 4351-61, (2007) (PubMed).

Bazil, Stefanová, Hilgert, Kristofová, Van?k, Bukovský, Horejsí: "Monoclonal antibodies against human leucocyte antigens. III. Antibodies against CD45R, CD6, CD44 and two newly described broadly expressed glycoproteins MEM-53 and MEM-102." in: Folia biologica, Vol. 35, Issue 5, pp. 289-97, (1990) (PubMed).

Details zu CD6

Target: CD6

Andere Bezeichnung: CD6 (CD6 Produkte)

Hintergrund: CD6 Molecule,CD6, also known as T12, is a member of the scavenger receptor superfamily found on T and B cell subsets, thymocytes, and acute lymphocytic leukemia cells (ALL). CD6 interacts with its ligand CD166/ALCAM (activated leukocyte cell adhesion molecule) and serves as a coreceptor for T cell activation and stabilizer of the immunological synapse. CD6-ALCAM mediated cell adhesion is also important for T cell proliferation. CD6 may exert some its functions via association with CD5, probably by fine-tuning CD5 signaling. Ligation of CD6 has antiapoptotic role in chronic lymphocytic leukemia B cells.,T12, TP120

Gen-ID: 923

UniProt: P30203