CD47 Antikörper (FITC)

Produktnummer ABIN192129

Produktdetails anti-CD47 Antikörper (FITC)

Target: CD47

Reaktivität: Human, Schwein, Non-Human Primate

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD47 Antikörper ist konjugiert mit FITC

Applikation: Flow Cytometry (FACS)

Spezifität: The antibody MEM-122 reacts with an extracellular epitope of CD47 (Integrin Associated Protein), a 50-55 kDa membrane adhesion molecule (thrombospondin receptor, immunoglobulin supergene family) expressed on leukocytes, platelets and erythrocytes. It is also expressed on epithelial cells, endothelial cells, fibroblasts and many tumor cell lines.

Kreuzreaktivität (Details): Non-Human Primates, Human, Porcine

Aufreinigung: Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: COS-7 (African green monkey) cells

Klon: MEM-122

Isotyp: IgM

Anwendungsinformationen

Applikationshinweise: Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.

Kommentare:

The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use. No reconstitution is necessary.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: No reconstitution is necessary.

Buffer: Stabilizing Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do not freeze.
Avoid prolonged exposure to light.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Referenzen für anti-CD47 Antikörper (FITC) (ABIN192129)

Smith, Patel, Seatter, Deehan, Brown, Brooke, Goodridge, Howard, Rigley, Harnett, Harnett: "A novel MyD-1 (SIRP-1alpha) signaling pathway that inhibits LPS-induced TNFalpha production by monocytes." in: Blood, Vol. 102, Issue 7, pp. 2532-40, (2003) (PubMed).

Details zu CD47

Target: CD47

Andere Bezeichnung: CD47 (CD47 Produkte)

Synonyme: IAP antikoerper, MER6 antikoerper, OA3 antikoerper, 9130415E20Rik antikoerper, AA407862 antikoerper, AI848868 antikoerper, AW108519 antikoerper, B430305P08Rik antikoerper, Itgp antikoerper, CD47/IAP antikoerper, alkaline phosphatase, intestinal antikoerper, CD47 molecule antikoerper, CD47 antigen (Rh-related antigen, integrin-associated signal transducer) antikoerper, Cd47 molecule antikoerper, ALPI antikoerper, CD47 antikoerper, Cd47 antikoerper

Hintergrund: CD47 Molecule,CD47 (integrin-associated protein, IAP) is an ubiquitously expressed cell surface transmembrane glycoprotein interacting with several integrins and regulating their functions. Engagement of CD47 by soluble ligands or counter receptors modulates various signaling pathways, such as activation of heterotrimeric G proteins. Binding secreted thrombospondin-1, CD47 counteracts graft vascularization. CD47 acts also as a ligand for CD172a (signal regulatory protein alpha, SIRP alpha), an immune inhibitory receptor on macrophages, this interaction prevents phagocytosis of CD47-positive cells. Moreover, CD47-CD172a system affects cell migration, B cell adhesion and T cell activation. CD47 is also involved in modulation of chondrocyte responses to mechanical signals, and promotes neuronal development, being especially abundant in synapse-rich regions of brain and retina.,IAP, OA3, MER6

Gen-ID: 961

UniProt: Q08722