CD21 Antikörper (PE)

Produktnummer ABIN192225

Produktdetails anti-CD21 Antikörper (PE)

Target: CD21 (CR2) (Complement Component (3d/Epstein Barr Virus) Receptor 2 (CR2))

Reaktivität: Human, Schwein, Rind (Kuh), Hund

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD21 Antikörper ist konjugiert mit PE

Applikation: Flow Cytometry (FACS)

Spezifität: The antibody LT21 reacts with an extracellular epitope of CD21 (CR2), a 145 kDa transmembrane glycoprotein (complement C3d receptor - C3dR) expressed on B lymphocytes, follicular dendritic cells, some epithelial cells and a subsets of T lymphocytes. It is not expressed on immature B cells.

Kreuzreaktivität (Details): Human, Porcine, Bovine, Canine (Dog)

Aufreinigung: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: IM9 human B-lymphoblastoid cell line

Klon: LT21

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.

Kommentare:

The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: No reconstitution is necessary.

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do not freeze.
Avoid prolonged exposure to light.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Referenzen für anti-CD21 Antikörper (PE) (ABIN192225)

Filatov, Krotov, Zgoda, Volkov: "Fluorescent immunoprecipitation analysis of cell surface proteins: a methodology compatible with mass-spectrometry." in: Journal of immunological methods, Vol. 319, Issue 1-2, pp. 21-33, (2007) (PubMed).

Faldyna, Samankova, Leva, Cerny, Oujezdska, Rehakova, Sinkora: "Cross-reactive anti-human monoclonal antibodies as a tool for B-cell identification in dogs and pigs." in: Veterinary immunology and immunopathology, Vol. 119, Issue 1-2, pp. 56-62, (2007) (PubMed).

Details zu CD21

Target: CD21 (CR2) (Complement Component (3d/Epstein Barr Virus) Receptor 2 (CR2))

Andere Bezeichnung: CD21 (CR2 Produkte)

Synonyme: C3DR antikoerper, CD21 antikoerper, CR antikoerper, CVID7 antikoerper, SLEB9 antikoerper, Cd21 antikoerper, cd21 antikoerper, CR1 antikoerper, CR1-L antikoerper, CR2 antikoerper, complement C3d receptor 2 antikoerper, complement C3b/C4b receptor 1 (Knops blood group) antikoerper, CR2 antikoerper, Cr2 antikoerper, CR1 antikoerper

Hintergrund: Complement C3d receptor 2,CD21 (complement receptor 2, CR2) binds C3 complement fragments, especially its breakdown fragments, which remain covalently attached to complement activating surfaces or antigen. CD21 has important roles in uptake and retention of immunocomplexes, survival of memory B cells and in development and maintenance of the humoral response to T-dependent antigens. CD21 also serves as a key receptor for Epstein-Barr virus binding and is involved in targeting prions to folicular dendritic cells and expediting neuroinvasion following peripheral exposure to prions. A soluble form of the CD21 (sCD21) is shed from the lymphocyte surface and retains its ability to bind respective ligands.,CR2, C3DR2, CVID7, SLEB9

Gen-ID: 1380

UniProt: P20023

Pathways: Komplementsystem