TUBB Antikörper

Produktnummer ABIN192340

Produktdetails anti-TUBB Antikörper

Target: TUBB (Tubulin, beta (TUBB))

Reaktivität: Human, Maus, Schwein, Pflanzen

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser TUBB Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Spezifität: The antibody TU-13 recognizes an epitope on N-terminal structural domain of beta-tubulin in various species.

Kreuzreaktivität (Details): Human, Porcine, Mouse, Plants

Aufreinigung: Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).

Reinheit: > 95 % (by SDS-PAGE)

Immunogen: beta-tubulin from porcine brain

Klon: TU-13

Isotyp: IgM

Anwendungsinformationen

Applikationshinweise: Western blotting: Recommended dilution: 1-2 μg/mL, reducing conditions.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do not freeze.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C. Do not freeze.

Referenzen für anti-TUBB Antikörper (ABIN192340)

Dráber, Vater, Böhm, Kuklova, Unger: "Inhibition of microtubule assembly in vitro by anti-tubulin monoclonal antibodies." in: FEBS letters, Vol. 262, Issue 2, pp. 209-11, (1990) (PubMed).

Dráber, Lagunowich, Dráberová, Viklický, Damjanov: "Heterogeneity of tubulin epitopes in mouse fetal tissues." in: Histochemistry, Vol. 89, Issue 5, pp. 485-92, (1988) (PubMed).

Details zu TUBB

Target: TUBB (Tubulin, beta (TUBB))

Andere Bezeichnung: beta-tubulin (TUBB Produkte)

Hintergrund: Tubulin beta,The microtubules are intracellular dynamic polymers made up of evolutionarily conserved polymorphic alpha/beta-tubulin heterodimers and a large number of microtubule-associated proteins (MAPs). The microtubules consist of 13 protofilaments and have an outer diameter 25 nm. Microtubules have their intrinsic polarity, highly dynamic plus ends and less dynamic minus ends. Microtubules are required for vital processes in eukaryotic cells including mitosis, meiosis, maintenance of cell shape and intracellular transport. Microtubules are also necessary for movement of cells by means of flagella and cilia. In mammalian tissue culture cells microtubules have their minus ends anchored in microtubule organizing centers (MTOCs). The GTP (guanosintriphosphate) molecule is an essential for tubulin heterodimer to associate with other heterodimers to form microtubule. In vivo, microtubule dynamics vary considerably. Microtubule polymerization is reversible and a populations of microtubules in cells are on their minus ends either growing or shortening –, this phenomenon is called dynamic instability of microtubules. On a practical level, microtubules can easily be stabilized by the addition of non-hydrolysable analogues of GTP (eg. GMPPCP) or more commonly by anti-cancer drugs such as Taxol. Taxol stabilizes microtubules at room temperature for many hours. Using limited proteolysis by enzymes both tubulin subunits can be divided into N-terminal and C-terminal structural domains. The beta-tubulin (relative molecular weight around 50 kDa) is counterpart of alpha-tubulin in tubulin heterodimer. It is coded by multiple tubulin genes and it is also posttranslationally modified. Heterogeneity of subunit is concentrated in C-terminal structural domain.,TUBB

Gen-ID: 81027

UniProt: Q9H4B7

Pathways: Microtubule Dynamics, M Phase