BUB1B Antikörper

Produktnummer ABIN1944865

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch BUB1B in WB. Er zeigt eine Reaktivität gegenüber Human und Maus und wurde in 5+ Publikationen erwähnt.

Kurzübersicht für BUB1B Antikörper (ABIN1944865)

Target: BUB1B (Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser BUB1B Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-BUB1B Antikörper

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: 1:1000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Lagerung: 4 °C,-20 °C

Referenzen für anti-BUB1B Antikörper (ABIN1944865)

Davenport, Fernandes, Harris, Neale, Goorha: "The mouse mitotic checkpoint gene bub1b, a novel bub1 family member, is expressed in a cell cycle-dependent manner." in: Genomics, Vol. 55, Issue 1, pp. 113-7, (1999) (PubMed).

Donadelli, Benatti, Remuzzi, Morigi, Gullans, Benigni, Remuzzi, Noris: "Identification of a novel gene--SSK1--in human endothelial cells exposed to shear stress." in: Biochemical and biophysical research communications, Vol. 246, Issue 3, pp. 881-7, (1998) (PubMed).

Taylor, Ha, McKeon: "The human homologue of Bub3 is required for kinetochore localization of Bub1 and a Mad3/Bub1-related protein kinase." in: The Journal of cell biology, Vol. 142, Issue 1, pp. 1-11, (1998) (PubMed).

Chan, Schaar, Yen: "Characterization of the kinetochore binding domain of CENP-E reveals interactions with the kinetochore proteins CENP-F and hBUBR1." in: The Journal of cell biology, Vol. 143, Issue 1, pp. 49-63, (1998) (PubMed).

Cahill, Lengauer, Yu, Riggins, Willson, Markowitz, Kinzler, Vogelstein: "Mutations of mitotic checkpoint genes in human cancers." in: Nature, Vol. 392, Issue 6673, pp. 300-3, (1998) (PubMed).

Details zu BUB1B

Target: BUB1B (Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B))

Andere Bezeichnung: BUB1B

Hintergrund: Essential component of the mitotic checkpoint. Required for normal mitosis progression. The mitotic checkpoint delays anaphase until all chromosomes are properly attached to the mitotic spindle. One of its checkpoint functions is to inhibit the activity of the anaphase-promoting complex/cyclosome (APC/C) by blocking the binding of CDC20 to APC/C, independently of its kinase activity. The other is to monitor kinetochore activities that depend on the kinetochore motor CENPE. Required for kinetochore localization of CENPE. Negatively regulates PLK1 activity in interphase cells and suppresses centrosome amplification. Also implicated in triggering apoptosis in polyploid cells that exit aberrantly from mitotic arrest. May play a role for tumor suppression.

Molekulargewicht: 119545 Da

Gen-ID: 701

UniProt: O60566