Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Lagerung
4 °C,-20 °C
Akgul, Turner, White, Edwards: "Functional analysis of the human MCL-1 gene." in: Cellular and molecular life sciences : CMLS, Vol. 57, Issue 4, pp. 684-91, (2000) (PubMed).
Bingle, Craig, Swales, Singleton, Zhou, Whyte: "Exon skipping in Mcl-1 results in a bcl-2 homology domain 3 only gene product that promotes cell death." in: The Journal of biological chemistry, Vol. 275, Issue 29, pp. 22136-46, (2000) (PubMed).
Umezawa, Maruyama, Inazawa, Imai, Takano, Hata: "Induction of mcl1/EAT, Bcl-2 related gene, by retinoic acid or heat shock in the human embryonal carcinoma cells, NCR-G3." in: Cell structure and function, Vol. 21, Issue 2, pp. 143-50, (1996) (PubMed).
Target
MCL-1 (MCL1)
(Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1))
Involved in the regulation of apoptosis versus cell survival, and in the maintenance of viability but not of proliferation. Mediates its effects by interactions with a number of other regulators of apoptosis. Isoform 1 inhibits apoptosis. Isoform 2 promotes apoptosis.