SHC1 Antikörper

Produktnummer ABIN1944925

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch SHC1 in WB und IHC. Er zeigt eine Reaktivität gegenüber Human, Maus und Ratte und wurde in 2+ Publikationen erwähnt.

Kurzübersicht für SHC1 Antikörper (ABIN1944925)

Target: SHC1 (SHC (Src Homology 2 Domain Containing) Transforming Protein 1 (SHC1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SHC1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails anti-SHC1 Antikörper

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: 1:1000. IHC: 1:50-1:100

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Lagerung: 4 °C,-20 °C

Referenzen für anti-SHC1 Antikörper (ABIN1944925)

Goshima, Kawamura, Fukumoto, Miura, Honma, Satoh, Wakamatsu, Yamamoto, Kimura, Nishikawa, Andoh, Iida, Ishikawa, Ito, Kagawa, Kaminaga, Kanehori, Kawakami, Kenmochi, Kimura, Kobayashi, Kuroita et al.: "Human protein factory for converting the transcriptome into an in vitro-expressed proteome,. ..." in: Nature methods, Vol. 5, Issue 12, pp. 1011-7, (2008) (PubMed).

Migliaccio, Mele, Salcini, Pelicci, Lai, Superti-Furga, Pawson, Di Fiore, Lanfrancone, Pelicci: "Opposite effects of the p52shc/p46shc and p66shc splicing isoforms on the EGF receptor-MAP kinase-fos signalling pathway." in: The EMBO journal, Vol. 16, Issue 4, pp. 706-16, (1997) (PubMed).

Details zu SHC1

Target: SHC1 (SHC (Src Homology 2 Domain Containing) Transforming Protein 1 (SHC1))

Andere Bezeichnung: Shc

Hintergrund: Signaling adapter that couples activated growth factor receptors to signaling pathways. Participates in a signaling cascade initiated by activated KIT and KITLG/SCF. Isoform p46Shc and isoform p52Shc, once phosphorylated, couple activated receptor tyrosine kinases to Ras via the recruitment of the GRB2/SOS complex and are implicated in the cytoplasmic propagation of mitogenic signals. Isoform p46Shc and isoform p52Shc may thus function as initiators of the Ras signaling cascade in various non-neuronal systems. Isoform p66Shc does not mediate Ras activation, but is involved in signal transduction pathways that regulate the cellular response to oxidative stress and life span. Isoform p66Shc acts as a downstream target of the tumor suppressor p53 and is indispensable for the ability of stress-activated p53 to induce elevation of intracellular oxidants, cytochrome c release and apoptosis. The expression of isoform p66Shc has been correlated with life span (By similarity). Participates in signaling downstream of the angiopoietin receptor TEK/TIE2, and plays a role in the regulation of endothelial cell migration and sprouting angiogenesis.

Molekulargewicht: 62822 Da

Gen-ID: 6464

UniProt: P29353

Pathways: RTK Signalweg, T-Zell Rezeptor Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, ER-Nucleus Signaling, Signaling Events mediated by VEGFR1 and VEGFR2