Immunohistochemistry (IHC), Immunofluorescence (IF), Western Blotting (WB), ELISA
Spezifität
Anti-BrdU Monoclonal Antibody was purified from ascites fluid by Protein A chromatography. This antibody reacts strongly with BrdU. Cross-reactivity is observed with CldU and IdU.
Aufreinigung
Protein A purified
Sterilität
Sterile filtered
Immunogen
Anti-BrdU monoclonal antibody was produced in mice by repeated immunizations prepared via immunizations with Bromodeoxyuridine-KLH followed by hybridoma development.
Usage: Anti-BrdU Antibody has been tested as suitable for immunoblot assays. Antibody may be suitable for additional immunoassays including immunofluorescence, flow cytometry and immunohistochemistry. Antibody will detect incorporated BrdU thymidine analog from replicated cells.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
Lot specific
Buffer
0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, sterile filtered
Handhabung
avoid freeze thaw cycles
Lagerung
4 °C,-20 °C
Informationen zur Lagerung
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.