RFP Antibody was prepared from egg yolks by a multi-step process which includes filtration, delipidation, salt fractionation and extensive dialysis against the buffer stated above. RFP Antibody was tested by western blot and immunoelectrophoresis.
Aufreinigung
Salt fractionation
Immunogen
The immunogen is a Red Fluorescent Protein (RFP) fusion protein corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma.
RFP
Reaktivität: Discosoma
WB, ELISA, IF
Wirt: Kaninchen
Polyclonal
HRP
Applikationshinweise
Approved: ELISA (1:10000), WB (1:1000 - 1:3000)
Usage: Anti-RFP is designed to detect recombinant RFP. This antibody can be used to detect RFP by immunoblotting. Use either alkaline phosphatase or peroxidase conjugated polyclonal anti-RFP to detect RFP or RFP containing proteins on western blots. Optimal titers for applications should be determined by the researcher. This product shows optimal performance by western blot. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Rekonstitution
Distilled water
Konzentration
Lot specific
Buffer
0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 1 % BSA
Handhabung
avoid freeze thaw cycles
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.