RFP Antikörper

Produktnummer ABIN1952571

Produktdetails anti-RFP Antikörper

Target: RFP (Red Fluorescent Protein (RFP))

Reaktivität: Discosoma

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser RFP Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Spezifität: Anti-RFP Monoclonal Antibody was purified from concentrated tissue culture supernate by Protein A chromatography. Expect reactivity against RFP and its variants: mCherry, tdTomato, mBanana, mOrange, mPlum, mOrange and mStrawberry.

Aufreinigung: Protein A purified

Sterilität: Sterile filtered

Immunogen: Anti-RFP monoclonal antibody is a Red Fluorescent Protein (RFP) fusion protein corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma.
ImmunogenType: Fusion protein

Klon: 8E5-G7

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: ELISA (1:20000 - 1:50000), WB (1:1000 - 1:3000),
Anti-RFP antibodies is designed to detect Red Fluorescent Protein and its variants. This antibody can be used to detect RFP by ELISA (sandwich or capture) for the direct binding of antigen. Biotin conjugated anti-RFP used in a sandwich ELISA with unconjugated anti-RFP is well suited to titrate RFP in solution. The detection antibody conjugated to biotin is subsequently reacted with streptavidin conjugated HRP. Fluorochrome conjugated anti-RFP can be used to detect RFP by immunofluorescence microscopy in cell expression systems and can detect RFP containing inserts. Significant amplification of signal is achieved using fluorochrome conjugated anti-RFP relative to the fluorescence of RFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated anti-RFP to detect RFP or RFP containing proteins on western blots. Optimal titers for applications should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2

Handhabung: avoid freeze thaw cycles

Lagerung: 4 °C

Informationen zur Lagerung: Short term 4°C, long term aliquot and store at -20°C, . Centrifuge product before removing cap. Only dilute immediately prior to use.

Details zu RFP

Target: RFP (Red Fluorescent Protein (RFP))

Andere Bezeichnung: RFP / Red Fluorescent Protein (RFP Produkte)