Ubiquitin Antikörper

Produktnummer ABIN1967211

Produktdetails anti-Ubiquitin Antikörper

Target: Ubiquitin

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Ubiquitin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Spezifität: Clone Ubi-1 was one of several clones which stained neurofibrillary tangles in frozen sectioned material strongly and specifically. Subsequent studies indicated that clone Ubi-1 is relatively insensitive to formalin fixation and so can be used on mildly fixed histological sections of human brain for studies of Alzheimer's disease. The antibody also works on paraffin embedded material. clone Ubi-1 also recognizes other ubiquitinated inclusion bodies such as the Lewy bodies of Parkinson's disease and the Pick bodies in Pick's disease in formalin fixed tissues. clone Ubi-1 will recognize human, bovine, chicken, Drosophila, and C. elegans in ELISA. clone Ubi-1 also works on Western blots and can be used to study ubiquitinated proteins. It has also been successfully used in competitive ELISA.

Aufreinigung: Affinity purified

Immunogen: Purified ubiquitin conjugated with glutaraldehyde to keyhole limpet hemocyanin. The clone was initially screened on ELISA of the immunogen, and subsequently tested on sections of Alzheimer brain. MCA-Ubi-1 was one of several clones which stained neurofibrillary tangles in frozen sectioned material strongly and specifically.

Type of Immunogen: Purified protein

Klon: Ubi-1-AP

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Approved: IF (1:500), IHC (1:1000), WB

Usage: Ubi-1 is excellent for the detection of ubiquitinated inclusions seen in human neurodegenerative diseases such as the neurofibrillary tangles of Alzheimer's disease. We recommend a starting dilution of 1:1000 for this purposes, using ABC or other enzymatic amplification procedures. For immunofluorescence try a dilution of 1:500.

Kommentare:

Target Species of Antibody: Mammal

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Liquid

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: 4°C or -20°C, Avoid freeze-thaw cycles.

Details zu Ubiquitin

Target: Ubiquitin

Andere Bezeichnung: Ubiquitin (Ubiquitin Produkte)

Synonyme: CG11624 antikoerper, DmUb antikoerper, DmUbi-p63E antikoerper, Dmel\\CG11624 antikoerper, Ub antikoerper, Ubi-P63e antikoerper, Ubi-p antikoerper, Ubi63E antikoerper, Ubi63p antikoerper, Ubip antikoerper, Ubiq antikoerper, Ubp antikoerper, ubi antikoerper, ubi-p63E antikoerper, ubi63E antikoerper, CG18282 antikoerper, CG32744 antikoerper, CR32744 antikoerper, DmUbi-p5E antikoerper, Dmel\\CG32744 antikoerper, ubiquitin antikoerper, si:dz180g5.5 antikoerper, si:busm1-180g5.5 antikoerper, LOC100194618 antikoerper, UBIQ antikoerper, Ubiquitin antikoerper, LOC100282068 antikoerper, Ubiquitin-63E antikoerper, Ubiquitin-5E antikoerper, transmembrane and ubiquitin-like domain containing 2 antikoerper, polyubiquitin antikoerper, Putative ubiquitin family protein antikoerper, ubiquitin antikoerper, ubiquitin B antikoerper, UBiQuitin antikoerper, Ubiquitin antikoerper, Ubi-p63E antikoerper, Ubi-p5E antikoerper, tmub2 antikoerper, LOC100194618 antikoerper, pco110026 antikoerper, LOC100135798 antikoerper, UBB antikoerper, ubq-1 antikoerper, LOC100190766 antikoerper

Pathways: Mitotic G1-G1/S Phases, Ubiquitin Proteasome Pathway