Dieser Phospholipase C gamma 1 Antikörper ist unkonjugiert
Applikation
Western Blotting (WB)
Spezifität
This antibody detects endogenous levels of PLC-gamma 1 only when phosphorylated at Tyrosine 783.
Aufreinigung
Affinity Chromatography using using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site
Immunogen
Peptide sequence around phosphorylation site of Tyrosine 783 (G-F-YP-V-E) derived from Human PLC-gamma-1 .
PLCG1
Reaktivität: Human
WB, ELISA, IHC
Wirt: Kaninchen
Polyclonal
unconjugated
Applikationshinweise
Suitable for use in Western blot (1: 500approx. 1: 1000). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Konzentration
1.0 mg/mL
Buffer
PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
Avoid repeated freezing and thawing.
Lagerung
-20 °C
Informationen zur Lagerung
Store the antibody (in aliquots) at -20 °C.
Target
Phospholipase C gamma 1 (PLCG1)
(phospholipase C, gamma 1 (PLCG1))
Phospholipase C gamma (PLCG) binds to receptor tyrosine kinases, e.g. EGFR, PDGFR, leading to phosphorylation of PLC-gamma at Tyrosine 771, 783 and 1245. Phosphorylation of Tyrosine 783 leads to enzymatic activation of PLC-gamma. PLC-gamma hydrolyzes phosphatidylinostitol-4,5-bisphosphate (PIP2), thus generating the second messengers inostitol-1,4,5-triphosphate (IP3) and diacylglycerol (DAG).Synonyms: 1-phosphatidylinositol-4, 5-bisphosphate phosphodiesterase gamma-1, PLC gamma1 Phospholipase C-II, PLC-148, PLC-II, PLC-gamma-1, PLC1, Phosphoinositide phospholipase C-gamma-1, Phospholipase C-gamma-1