ERK1/2 Antikörper (pThr187, pTyr204)
-
- Target Alle ERK1/2 (MAPK1/3) Antikörper anzeigen
- ERK1/2 (MAPK1/3) (Mitogen-Activated Protein Kinase 1/3 (MAPK1/3))
-
Bindungsspezifität
- pThr187, pTyr204
-
Reaktivität
- Human, Maus, Ratte
-
Wirt
- Kaninchen
-
Klonalität
- Polyklonal
-
Konjugat
- Dieser ERK1/2 Antikörper ist unkonjugiert
-
Applikation
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)
- Spezifität
- This antibody detects endogenous levels of p44/42 MAP Kinase only when phosphorylated at Tyrosine 204.
- Aufreinigung
- Affinity Chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
- Immunogen
- The antiserum was produced against synthesized phosphopeptide derived from Human p44/42 MAP Kinase around the phosphorylation site of Tyrosine 204 (T-E-Yp-V-A).
- Top Product
- Discover our top product MAPK1/3 Primärantikörper
-
-
- Applikationshinweise
-
Western Blot: 1/500approx. 1/1000. Immunohistochemistry: 1/50approx. 1/100. Immunofluorescence: 1/100approx. 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Protokoll
- 1. TETE HANNKEN, et al. (2000) Am Soc Nephrol 11: 1387-13972. Omar D. PerezNature et al. (2002) Biotechnology 20: 155 - 1623. Jingui Yu, et al. (2005) Anesth Analg 101: 315-3214. Hironobu Ihn et al. (2000) Immunology 165: 2149-2155
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
-
- Konzentration
- 1.0 mg/mL
- Buffer
- PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide as preservative and 50 % Glycerol as stabilizer.
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handhabung
- Avoid repeated freezing and thawing.
- Lagerung
- -20 °C
- Informationen zur Lagerung
- Store the antibody (in aliquots) at -20 °C.
-
- Target
- ERK1/2 (MAPK1/3) (Mitogen-Activated Protein Kinase 1/3 (MAPK1/3))
- Andere Bezeichnung
- ERK1 / ERK2 (MAPK1/3 Produkte)
- Synonyme
- erk1/2 antikoerper, mitogen-activated protein kinase antikoerper, erk1/2 antikoerper
- Hintergrund
- Both p44 and p42 MAP kinases (Erk1 and Erk2) function in a protein kinase cascade that plays a critical role in the regulation of cell growth and differentiation. Activation of MAP kinases occurs through phosphorylation of threonine and tyrosine (202 and 204 of human MAP kinase [Erk1] or 183 and 185 of rat Erk2) at the sequence T*EY* by a single upstream MAP kinase kinase (MEK). Both kinases are known to weakly autophosphorylate on tyrosine.Synonyms: ERK-1/ERK-2, Extracellular signal regulated kinase 1/2, Insulin stimulated MAP2 kinase, MAPK1/MAPK2, Microtubule associated protein 2 kinase, Mitogen activated protein kinase 1/2/3, P42/P44-MAPK
-