BIM Antikörper (pSer65, pSer69)

Produktnummer ABIN197056

Produktdetails anti-BIM Antikörper

Target: BIM (BCL2L11) (BCL2-Like 11 (Apoptosis Facilitator) (BCL2L11))

Bindungsspezifität: pSer65, pSer69

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser BIM Antikörper ist unkonjugiert

Applikation: Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Spezifität: This antibody detects endogenous levels of BIM only when phosphorylated at Serine 69.

Aufreinigung: Affinity Chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen: The antiserum was produced against synthesized phosphopeptide derived from Human BIM around the phosphorylation site of Serine 69 (Serine 65 in the Mouse sequence) (P-A-SP-P-G).

Anwendungsinformationen

Applikationshinweise: Immunofluorescence: 1/100-1/200. Immunohistochemistry on Paraffin-Embedded Sections: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store the antibody (in aliquots) at -20 °C.

Details zu BIM

Target: BIM (BCL2L11) (BCL2-Like 11 (Apoptosis Facilitator) (BCL2L11))

Abstract: BCL2L11 Produkte

Synonyme: bim antikoerper, BAM antikoerper, BIM antikoerper, BOD antikoerper, 1500006F24Rik antikoerper, Bim antikoerper, Bod antikoerper, BimL antikoerper, BIMEL antikoerper, BCL2-like 11 antikoerper, BCL2 like 11 antikoerper, BCL2-like 11 (apoptosis facilitator) antikoerper, bcl2l11 antikoerper, BCL2L11 antikoerper, Bcl2l11 antikoerper

Hintergrund: Bim, Bcl-2 interacting mediator of cell death, is a pro-apoptotic protein belonging to the Bcl2 family of proteins containing a Bcl2 homology domain 3 (BH3). It is proapoptotic and exerts its effects by interacting with prosurvival members of the Bcl2 family like Bcl2, BclxL and Bclw. Bim is sequestered in an inactive conformation through binding to the microtubule-associated dynein motor complex. Certain apoptotic stimuli release Bim from microtubules, allowing inhibitory binding to anti-apoptotic Bcl-2 family members and subsequent iniation of apoptosis.Synonyms: BCL2L11, BIM, Bcl2-L-11, Bcl2-interacting mediator of cell death, BimEL, BimL, BimS

Gen-ID: 10018

NCBI Accession: NP_001191035

UniProt: O43521

Pathways: PI3K-Akt Signalweg, Neurotrophin Signalübertragung, Tube Formation, Positive Regulation of Endopeptidase Activity