This antibody detects endogenous levels of MARCKS only when phosphorylated at Serine 158.
Aufreinigung
Affinity Chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human MARCKS around the phosphorylation site of serine 158 (R-F-SP-F-K).
MARCKS
Reaktivität: Ratte
ELISA, IF (cc), IF (p), IHC (p), IHC (fro)
Wirt: Kaninchen
Polyclonal
unconjugated
Applikationshinweise
Western Blot: 1/500-1/1000. Immunofluorescence: 1/100-1/200. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Konzentration
1.0 mg/mL
Buffer
PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
Avoid repeated freezing and thawing.
Lagerung
-20 °C
Informationen zur Lagerung
Store the antibody (in aliquots) at -20 °C.
Target
MARCKS
(Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS))
80K-L antikoerper, MACS antikoerper, PKCSL antikoerper, PRKCSL antikoerper, KINC antikoerper, Macs antikoerper, marcks antikoerper, MGC89127 antikoerper, MARCKS antikoerper, macs antikoerper, wu:fb55c06 antikoerper, wu:fb92d04 antikoerper, wu:fc19b04 antikoerper, wu:fc44a10 antikoerper, zgc:110235 antikoerper, fc34g03 antikoerper, fc38c06 antikoerper, wu:fc34g03 antikoerper, wu:fc38c06 antikoerper, zgc:109978 antikoerper, myristoylated alanine rich protein kinase C substrate antikoerper, myristoylated alanine-rich protein kinase C substrate antikoerper, methyl binding domain106 antikoerper, myristoylated alanine-rich protein kinase C substrate b antikoerper, myristoylated alanine-rich protein kinase C substrate S homeolog antikoerper, myristoylated alanine-rich protein kinase C substrate a antikoerper, MARCKS antikoerper, Marcks antikoerper, marcks antikoerper, mbd106 antikoerper, marcksb antikoerper, marcks.S antikoerper, marcksa antikoerper
Hintergrund
MARCKS, (Myristoylated Alanine-Rich C Kinase Substrate), is a member of a family of calmodulin binding proteins and is a major substrate for phosphorylation by protein kinase C (PKC). The phosphorylation of Ser152/156 can be used as a measure of PKC activation. Phosphorylation of Ser152/156 modulates the binding of MARCKS to calmodulin.Synonyms: MACS, Myristoylated alanine-rich C-kinase substrate, PRKCSL