Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Spezifität
PKCδ antibody detects endogenous levels of PKC-delta only when phosphorylated at Serine 645.
Aufreinigung
Affinity Chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from Human PKC-delta around the phosphorylation site of Serine 645 (R-L-Sp-Y-S).
Western Blot: 1: 500approx. 1: 1000. Immunohistochemistry: 1: 50approx. 1: 100. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Konzentration
1.0 mg/mL
Buffer
PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
MAY1 antikoerper, PKCD antikoerper, nPKC-delta antikoerper, AI385711 antikoerper, D14Ertd420e antikoerper, PKC[d] antikoerper, PKCdelta antikoerper, Pkcd antikoerper, fb09d10 antikoerper, fv43b11 antikoerper, prkcd antikoerper, wu:fb09d10 antikoerper, wu:fv43b11 antikoerper, zgc:56558 antikoerper, PKC-delta antikoerper, may1 antikoerper, npkc-delta antikoerper, pkc-delta2 antikoerper, pkcd antikoerper, tpa-1 antikoerper, protein kinase C delta antikoerper, protein kinase C, delta antikoerper, protein kinase C, delta a antikoerper, protein kinase C delta L homeolog antikoerper, PRKCD antikoerper, Prkcd antikoerper, prkcda antikoerper, prkcd.L antikoerper, prkcd antikoerper
Hintergrund
Protein Kinase C delta (PKC delta) is a 78 kDa member of the novel group (nPKCs: sensitive to diacylglycerol, phosphatidylserine, and phorbol esters) of the PKC family of serine/threonine kinases that are involved in a wide range of physiological processes including mitogenesis, cell survival and transcriptional regulation. PKC delta is an ubiquitously expressed PKC isozyme that has been implicated in the regulation of multiple cellular processes including cell cycle progression and apoptosis. Autophosphorylation of serine 664 (serine 662 in mouse and rat) contributes to PKC delta activity. Serum dependent phosphorylation of serine 664 is mediated by mTOR pathway, is protected from dephosphorylation by phosphorylated threonine 505 in the activation loop, and is implicated in prolactin signaling.Synonyms: PKC delta, PKC-delta, PKCD, Protein kinase C delta type