Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Spezifität
MEK2 antibody detects endogenous levels of total MEK2 protein.
Aufreinigung
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Immunogen
The antiserum was produced against synthesized non-phosphopeptide derived from human MEK2 around the phosphorylation site of threonine 394 (P-G-TP-P-T).
MAP2K2
Reaktivität: Human
WB, IHC, ELISA, IF
Wirt: Kaninchen
Polyclonal
unconjugated
Applikationshinweise
Western Blot: 1: 500approx. 1: 1000. Immunohistochemistry: 1: 50approx. 1: 100. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Konzentration
1.0 mg/mL
Buffer
PBS(without Mg2+ and Ca2+), pH 7.4 containing 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
Avoid repeated freezing and thawing.
Lagerung
-20 °C
Target
MEK2 (MAP2K2)
(Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2))
Microtubule associated protein kinase kinase 2 (MEK2 or MAPKK2) is a member of a family of tyrosine/threonine protein kinases that activate the ERK 1 & 2/MAPK enzymes by phosphorylating both residues within the threonine/glutamic acid/tyrosine (TEY) motif in the activation loop. MEK 1 & 2 are also activated by dual phosphorylation, which occurs on serines 218 and 222, in the activation loop of the MEKs. Threonine 394 of MEK2 is phosphorylated by ERK 2, which serves as a negative feedback loop by suppressing activation of MEK2.Synonyms: Dual specificity mitogen-activated protein kinase kinase 2, ERK activator kinase 2, MAP kinase kinase 2, MAPK/ERK kinase 2, MEK2, MKK2