BAD Antikörper (Ser112)

Produktnummer ABIN197208

Produktdetails anti-BAD Antikörper

Target: BAD (BCL2-Associated Agonist of Cell Death (BAD))

Bindungsspezifität: Ser112

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser BAD Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Spezifität: BAD antibody detects endogenous levels of total BAD protein.

Aufreinigung: Affinity-chromatography using epitope-specific immunogen

Immunogen: The antiserum was produced against synthesized non-phosphopeptide derived from human BAD around the phosphorylation site of serine 112 (H-S-SP-Y-P).

Anwendungsinformationen

Applikationshinweise: Western Blot: 1: 500approx. 1: 1000. Immunohistochemistry: 1: 50approx. 1: 100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS(without Mg2+ and Ca2+), pH 7.4 containing 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Details zu BAD

Target: BAD (BCL2-Associated Agonist of Cell Death (BAD))

Abstract: BAD Produkte

Synonyme: bad antikoerper, fa01b12 antikoerper, wu:fa01b12 antikoerper, wu:fa96d04 antikoerper, BAD antikoerper, BBC2 antikoerper, BCL2L8 antikoerper, AI325008 antikoerper, Bbc2 antikoerper, BCL2-associated agonist of cell death b antikoerper, BCL2 associated agonist of cell death antikoerper, BCL2-associated agonist of cell death antikoerper, badb antikoerper, BAD antikoerper, Bad antikoerper

Hintergrund: Bad is a member of the Bcl2 family and acts to promote apoptosis by forming heterodimers with the survival proteins Bcl2 and BclxL, thus preventing them from binding with BAX. Bad is found on the outer mitochondrial membrane and, once phosphorylated in response to growth stimuli, translocates to the cytoplasm. The phosphorylation status of Bad represents a key checkpoint for death or cell survival. JNK-induced phosphorylation of BAD serine 128 promotes the apoptotic role of Bad by opposing the inhibitory effect of growth factor on Bad-mediated apoptosis. Cdc2-induced phosphorylation of Bad serine 128 has an inhibitory effect on its interaction with 14-3-3 proteins. The latter interaction is critical for Bad phosphorylation at serine 155, a site within the BH3 domain that leads to the release of BclxL and the promotion of cell survival. Alternative splicing of this gene results in two transcript variants which encode the same isoform.Synonyms: BAD, BBC6, BCL2L8, Bcl-2-binding component 6, Bcl-2-like protein 8, Bcl-XL/Bcl-2-associated death promoter, Bcl2 antagonist of cell death, Bcl2-L-8

Gen-ID: 572

NCBI Accession: NP_004313

UniProt: Q92934

Pathways: MAPK Signalweg, PI3K-Akt Signalweg, RTK Signalweg, Apoptose, Fc-epsilon Rezeptor Signalübertragung, Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Hepatitis C, CXCR4-mediated Signaling Events