Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Spezifität
G3BP-1 antibody detects endogenous levels of total G3BP-1 protein.
Aufreinigung
Affinity-chromatography using epitope-specific immunogen
Immunogen
The antiserum was produced against synthesized non-phosphopeptide derived from human G3BP-1 around the phosphorylation site of serine 232 (S-S-SP-P-A).
G3BP1
Reaktivität: Human
WB, IP
Wirt: Kaninchen
Polyclonal
unconjugated
Applikationshinweise
Western Blot: 1: 500approx. 1000. Immunohistochemsistry: 1: 50approx. 1: 100. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Konzentration
1.0 mg/mL
Buffer
PBS(without Mg2+ and Ca2+), pH 7.4 containing 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
Avoid repeated freezing and thawing.
Lagerung
-20 °C
Target
G3BP1
(GTPase Activating Protein (SH3 Domain) Binding Protein 1 (G3BP1))
G3BP antikoerper, HDH-VIII antikoerper, fj17h05 antikoerper, zgc:56034 antikoerper, wu:fj17h05 antikoerper, g3bp antikoerper, MGC53271 antikoerper, G3BP1 antikoerper, G3bp antikoerper, RGD1310666 antikoerper, AI849976 antikoerper, B430204O07 antikoerper, C87777 antikoerper, mKIAA4115 antikoerper, G3BP stress granule assembly factor 1 antikoerper, GTPase activating protein (SH3 domain) binding protein 1 antikoerper, GTPase activating protein (SH3 domain) binding protein 1 L homeolog antikoerper, Ras-GTPase-activating protein SH3-domain-binding protein antikoerper, G3BP1 antikoerper, g3bp1 antikoerper, g3bp1.L antikoerper, LOC100304885 antikoerper, G3bp1 antikoerper
Hintergrund
G3BP is one of the DNA-unwinding enzymes which prefers partially unwound 3'-tailed substrates and can also unwind partial RNA/DNA and RNA/RNA duplexes in an ATP-dependent fashion. This enzyme is a member of the heterogeneous nuclear RNA-binding proteins and is also an element of the Ras signal transduction pathway. It binds specifically to the Ras-GTPase-activating protein by associating with its SH3 domain. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.Synonyms: ATP-dependent DNA helicase VIII, G3BP-1, GAP SH3 domain-binding protein 1, Ras GTPase-activating protein-binding protein 1, hDH VIII